海洋科学2016，Vol.40Issue(5)：49-56,8.DOI:10.11759//hykx20141219001
糙海参精子的发生及超微形态结构
Spermatogenesis and sperm morphology of sea cucumber, Holothuria scabra
摘要
Abstract
The wild sea cucumber,Holothuria scabra,was sampled monthly at Hainan Island from November 2014 to April 2015 to understand spermatogenesis and sperm ultrastructure. The testis were removed and fixed in situ to prepare histological sections. The sections were observed under a scanning electron microscope,a transmission electron microscope,and a light microscope. The results showed that spermatogenesis inH. scabra included five stages of spermatogonium,primary spermatocyte,secondary spermatocyte,spermatidand spermatozoon. Differentiation of spermatids was subdivided into three stages of early spermatid (formation of the proacrosomal vesicle at the front of the nucleus),mid-spermatid (periacrosomal material in the acrosomal cavity),late spermatid (cytoplasm shrinking and formation of flagellum) and spermatozoon. The mature spermatozoon was flagellated,approximately 60μm long,and consisted of a head,mid-pieceand tail. The head was spherical,and the acrosome was a U-shaped cystid. The periacrosomal material space was crescent shaped,and the chromatin in the acrosome was condensed into a nucleus. Large mitochondria and the centriole complex were located in the mid-piece of the spermatozoon,and the flagellum in the tail had the typical “9 + 2” microtubular structure. These results will provide a theoretical reference for reproductive biology and artificial breeding of the sea cucumber.关键词
糙海参(Holothuria scabra)/精子发生/形态/超微结构Key words
Holothuria scabra/spermatogenesis/morphology/ultrastructure分类
生物科学引用本文复制引用
杨学明,吴明灿,张立,杨琼,潘英,陈福艳..糙海参精子的发生及超微形态结构[J].海洋科学,2016,40(5):49-56,8.基金项目
国家自然科学基金资助项目(31260636) （31260636）
广西自治区直属公益性科研院所资助项目(GXIF-2014-008)@@@@National Natural Science Foundation of China, No.31260636 （GXIF-2014-008）
The public welfare scientific research projects directly under Guangxi municipality, GXIF-2014-008。 （）
