海洋科学2016，Vol.40Issue(5)：57-65,9.DOI:10.11759/hykx20150706002
不同培养条件对三角褐指藻生长及其生物活性成分积累的影响
Effects of different culture conditions on growth and accumu-lation of bioactive compounds byPhaeodactylum tricornutum
摘要
Abstract
In this study, the effects of mL1 and ASW media containing NaNO3, CO(NH2)2, or NH4HCO3 on the growth and accumulation of bioactive compounds [fucoxanthin, chrysolaminaran, and eicosapentaenoic acid (EPA)] by the marine diatomPhaeodactylum tricornutum were investigated; and the total lipid content and fatty acid com-position were analyzed. The results showed that maximum biomass concentration in mL1 medium with urea as the nitrogen source was 3.7 g/L. Fucoxanthin content initially increased and then decreased during culture under dif-ferent conditions, and the highest quantities that accumulated were 13.27 mg/g (NaNO3), 13.23 mg/g (CO(NH2)2), and 13.89 mg/g (NH4HCO3) in mL1 medium and 13.2 mg/g (NaNO3), 14.92 mg/g (CO(NH2)2), and 13.6 mg/g (NH4HCO3) in ASW medium, indicating that the nitrogen source had a weak effect on fucoxanthin accumulation. Chrysolaminaran content increased gradually during the culture period, and the highest contents were 9.82 mg/g (NH4HCO3) in mL1 and 8.59 mg/g (NaNO3) in ASW on day 15. The total lipid content changed slightly under the different conditions, and the maximum values were 24.18% (NH4HCO3) in mL1 and 23.79% (NaNO3) in ASW, which were observed during the stationary phase. The fatty acid profiles were myristic acid (C14: 0), palmitic acid (C16: 0), palmitoleic acid (C16: 1), stearic acid (C18: 0), oleic acid (C18: 1), linoleic acid (C18: 2), eicosaenoic acid (C20: 1), tetracosanoic acid (C24: 0), and EPA (C20: 5). The EPA content decreased gradually as culture time was extended. Urea was the optimal nitrogen source to accumulate EPA.关键词
三角褐指藻/生物质质量浓度/岩藻黄素/金藻昆布糖/二十碳五烯酸Key words
Phaeodactylum tricornutum/biomass concentration/fucoxanthin/chrysolaminaran/eicosapentaenoic acid分类
生物科学引用本文复制引用
张文源,高保燕,李爱芬,张成武..不同培养条件对三角褐指藻生长及其生物活性成分积累的影响[J].海洋科学,2016,40(5):57-65,9.基金项目
国家“863”项目(2013AA065805) （2013AA065805）
国家自然科学基金项目(31170337) （31170337）
广东省低碳专项(2011-051) （2011-051）
珠海市科技重大项目(PB20041018) （PB20041018）
珠海市科技攻关项目(PC20081008)@@@@National High Technology Research and Development Pro-gram of China (863Program), No.2013AA065805 （PC20081008）
Natural Science Foundation of China, No.31170337 （）
the Special Program for Low-Carbon, Reform and Development Commission of Guangdong Province, NO.2011-051 （）
Scien-tific and Technological Key Program of Zhuhai, No.PB20041018 （）
Scien-tific and Technological Project Zhuhai, No. PC20081008。 （）
