临床神经外科杂志2016，Vol.13Issue(4)：263-266,271,5.DOI:10.3969/j.issn.1672-7770.2016.04.007
FRK 通过 FAK 信号通路抑制脑胶质瘤细胞迁移与侵袭作用的研究
FRK inhibits human glioma cells invasion and migration through FAK regulation
摘要
Abstract
Objective To explore the mechanism of the inhibitory effect of fyn-related kinase ( FRK) on the glioma cells migration and invasion through focal adhesion kinase ( FAK) signaling pathways .Methods Western blot was used to examine the transfection efficiency of FRK plasmid . The effect of FRK over-expression or FAK down-regulation on the glioma U251 cells migration and invasion ability was examined by wound healing assay and transwell invasion assay respectively .The effect of FRK over-expression on the protein level of FAK , P-FAK, and the influence of FAK down-regulation on FRK protein level were analyzed by WB .Results The FRK plasmid was transfected into glioma U251 cells successfully , and FRK over-expression decreased the glioma cells migration and invasion .The efficiency of FAK reached to about 70%, and FAK down-regulation decreased the migratory and invasive ability of glioma U 251 cells.FRK over-expression decreased the protein level of P-FAK significantly , but had no effect on the total protein levels of FAK .In addition, the protein levels of FRK had no change after FAK down-regulation .Conclusion FRK may regulate the migration and invasion of the glioma cells by dephosphorylating the focal adhesion kinase .关键词
胶质瘤/迁移与侵袭/酪氨酸相关激酶/粘着斑激酶Key words
glioma/fyn-related kinase/migration and invasion/focal adhesion kinase分类
医药卫生引用本文复制引用
王军,石琼,张春亭,金戈,张道为,宋旭,周秀萍,于如同..FRK 通过 FAK 信号通路抑制脑胶质瘤细胞迁移与侵袭作用的研究[J].临床神经外科杂志,2016,13(4):263-266,271,5.基金项目
国家自然科学基金 （）
