临床神经外科杂志2016，Vol.13Issue(4)：276-280,5.DOI:10.3969/j.issn.1672-7770.2016.04.010
急性乙醇中毒对大鼠创伤性脑损伤后GFAP、AQP4表达的影响
Effect of expression of GFAP and AQP4 in rats with traumatic brain injury by acute ethanol intoxication
摘要
Abstract
Objective To investigate the effect of acute ethanol intoxication ( AEI ) on the expression of GFAP and AQP4 in serum and brain tissue after traumatic brain injury ( TBI) in rats. The underlying mechanism was also investigated .Method 45 rats according to body weight were randomly assigned to 3 groups, as experimental group 1,2 and control group, each group has 15 rats.Rats in experiment group 1,2 were given 30%, 60% ethanol separately (1 ml /100g) by stomach irrigation ( ethanol diluted with distilled water ) , the control group was given the same dose of distilled water .Rats in each group underwent improved Feeney 's free falling epidural hitting for traumatic brain injury model 1 h after ethanol administration .After TBI 1 h, 6 h, 24 h, respectively from each experimental group and control group were randomly selected from five rats blood , subsequent high-speed centrifugation , the levels of GFAP and AQP4 in serum was determinated . The rats were killed after blood was drawn and the brain was quickly removed , and the levels of GFAP and AQP4 in brain tissue was determined .Results ( 1 ) Compared with the control group , the expression of AQP4 and GFAP in serum and brain tissues of 1, 2 group were significantly increased (all P<0.05).(2) The expressions of AQP4 and GFAP in serum and brain tissues of the group 2 were significantly higher than those in group 1 ( all P<0 .05 ) .( 3 ) The expression of GFAP and AQP4 in the serum and brain tissue of the three groups was more obvious with the prolonging of the time of injury .Conclusions Acute ethanol intoxication can increase the severity of TBI in rats, and the higher the concentration of ethanol in blood , the longer after the injury,the more after the TBI .关键词
急性乙醇中毒/创伤性脑损伤/胶质纤维酸性蛋白/水通道蛋白4Key words
acute ethanol intoxication/traumatic brain injury/GFAP/AQP4分类
医药卫生引用本文复制引用
黄培赞,赵金兵,赵鹏来,章文斌,罗正祥,杨伦先,张岩松..急性乙醇中毒对大鼠创伤性脑损伤后GFAP、AQP4表达的影响[J].临床神经外科杂志,2016,13(4):276-280,5.基金项目
国家自然科学青年基金（81301049）；江苏省自然科学基金 （）
