林业科学2016，Vol.52Issue(7)：87-95,9.DOI:10.11707/j.1001-7488.20160711
广西良凤江国家森林公园植物内生拟盘多毛孢属真菌种类多样性调查
Survey of Species Diversity in the Endophytic Fungal Genus Pestalotiopsis in Liangfengjiang National Forest Park,Guangxi,China
摘要
Abstract
Obiective]In order to understand composition of endophytic Pestalotiopsis and provide the solid foundation for research on metabolites of endophytic Pestalotiopsis. A survey on diversity of endophytic Pestalotiopsis of 16 plant species of Caesalpiniaceae,Magnoliaceae and Myrtaceae were carried out in Nanning Liangfengjiang Forest Park of Guangxi. [Method]The tissue isolation and molecular techniques were used to preliminarily analyze the correlations between endophytic Pestalotiopsis and plant species,plant parts and seasons from or in which the fungus was isolated.[Result] Results showed that 89 isolates of endophytic Pestalotiopsis were isolated from 16 plant species of Caesalpiniaceae,Magnoliaceae and Myrtaceae,and among the isolates 11 species were identified based on morphological characters. Eight, six, eight and six endophytic Pestalotiopsis species were respectively collected from Melaleuca leucadendra ( Myrtaceae),Syzygium polypetaloideum ( Myrtaceae),Peltophorum tonkinense ( Caesalpiniaceae),and Saraca chinensis ( Caesalpiniaceae) ,in which the most endophytic Pestalotiopsis species colonized,of which the highest isolation frequency was 2. 86% from Peltophorum tonkinense. It was also found that colonization frequencies of endophytic Pestalotiopsis were higher on twigs than leaves for most of plant species,and higher in autumn than in other seasons. In comparison, Shannon-Wiener ’s diversity index of endophytic Pestalotiopsis was the highest in Caesalpiniaceae (2. 235 3),the second in Myrtaceae (2. 163 5) and the lowest in Magnoliaceae (1. 863 7). [Conclusion]It indicated that endophytic Pestalotiopsis had preference colonizing in Peltophorum tonkinense,Saraca chinensis,Erythrophleum fordii, and Melaleuca leucadendra and the colonization frequencies were higher in twigs and its colonization frequencies varied in different seasons,among which the highest frequencies were in autumn.关键词
拟盘多毛孢属/定殖率/多样性/苏木科/木兰科/桃金娘科Key words
Pestalotiopsis/colonization/diversity/Caesalpiniaceae/Magnoliaceae/Myrtaceae分类
农业科技引用本文复制引用
宋利沙,韦继光,云朝光,杨秀好,罗基同,黄松殿..广西良凤江国家森林公园植物内生拟盘多毛孢属真菌种类多样性调查[J].林业科学,2016,52(7):87-95,9.基金项目
国家自然科学基金项目(30970022，31270076) （30970022，31270076）
广西科学研究与技术开发计划项目(桂科重14121002－4－3) （桂科重14121002－4－3）
浙江省科技计划项目(2012C12009－5)。 （2012C12009－5）
