山东医药2016，Vol.56Issue(29)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.29.001
白藜芦醇对人急性T淋巴细胞白血病的体外治疗作用及其机制
Therapeutic effect and mechanism of resveratrol on human T lymphatic leukemia cells
摘要
Abstract
Objective To investigate the therapeutic effect and mechanism of resveratrol (Res) on human T lymphatic leukemia cells (Hut78) .Methods Human T lymphocytic leukemia (Hut78) cells were selected as the target cells, which were treated with 6.25, 12.5, 25, 50, 100 and 200μmol/L Res, respectively.CCK-8 assay was used to detect the cell inhibition rate of Hut78 cells which were treated with 100μmol/L Res for 12, 24 and 48 h, respectively.Flow cytometry was used to detect the apoptosis of Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 12 and 24 h.Western blotting was used to detect the expression of C-myc protein in Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 48 h. Results Resveratrol whose dose was greater than 6.25μmol/L significantly inhibited the proliferation of Hut78 cells, and was time-and dose-dependent (all P<0.01).Res induced the apoptosis of Hut 78 cells, which was dose-dependent (all P<0.01). Res own-regulated the expression of C-myc protein which was in a dose-dependent manner (all P<0.01).Conclusion Res-veratrol has significant anti-leukemia effect on human T lymphatic leukemia, and its mechanism may be associated with the down-regulated expression of C-myc protein.关键词
T淋巴细胞白血病/白藜芦醇/细胞增殖/细胞凋亡/C-myc蛋白Key words
T lymphatic leukemia/resveratrol/cell proliferation/apoptosis/C-myc protein分类
医药卫生引用本文复制引用
马丽君,姜中兴,田文亮,何琛,郭荣,孙慧,孙玲,李英梅..白藜芦醇对人急性T淋巴细胞白血病的体外治疗作用及其机制[J].山东医药,2016,56(29):1-3,3.基金项目
国家自然科学基金资助项目（81070445）；河南省医学科技攻关项目（20090349）。 （）
