山东医药2016，Vol.56Issue(29)：4-6,3.DOI:10.3969/j.issn.1002-266X.2016.29.002
丹参总酚酸对刀豆蛋白A所致小鼠急性肝损伤的保护作用及机制
Protective effect of salvianolic acids on acute liver injury caused by concanavalin A in mice
摘要
Abstract
Objective To investigate the protective effect of salvianolic acids on acute liver injury caused by con-canavalin A (ConA) in mice and its mechanism.Methods Male C57BL/6 (B6) mice were randomly divided into four groups:the normal control group, which received saline only; salvianolic acids-treated group, which received salvianolic acids only;ConA-treated group, which was administered with ConA (40 mg/kg) via tail vein injection;and the salvianolic acids+ConA-treated group, which received salvianolic acids at a dose of 50 mg/kg 30 min prior to ConA injection.After 16-hour intervention, the liver function was analyzed by fully automatic biochemical analyser, pathologic changes were ob-served by HE staining, and the relevant inflammatory mediators ( extracellular histones, IL-1 , IL-6, IL-18 and TNF-α) were evaluated by ELISA and Luminex.Results The administration of ConA to mice caused severe liver injury, as evi-denced by significantly increased ALT and AST levels, necrosis and apoptosis of liver cells and inflammatory cell infiltra-tion.Compared with the ConA-treated mice, salvianolic acids plus ConA-treated mice showed a significantly decreasing trend of ALT, AST, as well as the reduced liver injury.Besides, the concentrations of extracellular histones, IL-1 , IL-6, IL-18 and TNF-αwere significantly decreased in the ConA-treated group as compared with those of the normal control group and salvianolic acids-treated group (all P<0.05).Compared with the ConA-treated group, all indexes of the salvianolic acids+ConA-treated group were decreased (all P<0.01), but were higher than those of the normal control group and salvianolic acids-treated group (all P<0.01).Conclusion Salvianolic acids have significant protective effect on ConA-in-duced liver injury in mice, likely due to its ability of decreasing the levels of extracellular histones and various cytokines.关键词
肝损伤/丹参总酚酸/刀豆蛋白A/炎症因子Key words
liver injury/salvianolic acids/concanavalin A/inflammatory factors分类
医药卫生引用本文复制引用
何佳丽,刘焱,温韬..丹参总酚酸对刀豆蛋白A所致小鼠急性肝损伤的保护作用及机制[J].山东医药,2016,56(29):4-6,3.基金项目
国家自然科学基金资助项目（81372094）；北京市卫生系统高技术人才培养项目－学科骨干（2013-3-077）。 （）
