水生态学杂志2016，Vol.37Issue(4)：16-22,7.DOI:10.15928/j.1674-3075.2016.04.003
光照强度对香溪河水华类型影响的模拟实验
Effect of Light Intensity on Algal Blooms in the Lower Xiangxi River
摘要
Abstract
Chlorella vulgaris is a common single-celled green algae,and accounts for a large portion of green algae blooms.Under the same light conditions,Chlorella vulgaris competes with blue-green algae for nitrogen and phos-phorous and,to some degree,inhibits the growth of blue-green algae.Thus,studying the growth characteristic of Chlorella vulgaris can help predict the type of algae bloom most likely to occur.This study investigated the effects of varying light intensity on the growth of Chlorella to provide a theoretical basis for predicting algal blooms in the Xiangxi River near its outlet to Three Gorges Reservoir.Chlorella vulgaris was obtained from the Xiangxi River,in-cubated in a 40 L square plastic container after purification and 1 0 ml of this solution was added to each incubator. The culture solution was BG-1 1 ,diluted 25 times with water to give 0.285 mgP/L,9.48 mgN /L and pH =7.3 -7.8,conditions similar to the lower Xiangxi River.The incubator was maintained at 24℃,covered by a cloth for shade,and fresh nutrient solution was added every 24 hours.A vacuum pump was used for aeration and the aera-tion rate was 1 0%.Seven light intensities (0 lx,800 lx,1 500 lx,2 400 lx,3 200 lx,4 500 lx,6 000 lx)were used and the experiment was run in duplicate.The experiment lasted for 1 2 days and samples were collected at 8 a. m.each day to determine the optical density (OD),chlorophyll a,dissolved total phosphorous (DTP)and dis-solved total nitrogen (DTN).Results show:A long period green algal bloom tends to develop at a light intensity of 800 -1 500 lx and the chance of transforming to a blue-green algae bloom is low;a short period green algal bloom tends to occur at a light intensity of 3 200 -4 500 lx and a blue-green algae bloom is likely at 6 000 lx.When the light intensity is <3 200 lx,there is no need to take measures to control blue-green algae;when the light intensity is >4 500 lx,it is necessary to take action to prevent and control cyanobacteria blooms.We also found that Chlo-rella vulgaris biomass,under high light intensity,is estimated more accurately by measuring chlorophyll a rather than optical density.This experiment was run under specific nutrient conditions and should be used for algal bloom prediction only in water bodies similar to Xiangxi River.关键词
小球藻/水华/光照强度/香溪河Key words
Chlorella vulgaris/algal bloom/illumination intensity/the Xiangxi River分类
生物科学引用本文复制引用
陈钊,刘德富,杨正健,崔玉洁..光照强度对香溪河水华类型影响的模拟实验[J].水生态学杂志,2016,37(4):16-22,7.基金项目
973计划前期研究课题（2014CB460601）；国家国际合作项目（2014DFE70070）；国家自然科学基金项目（51509086）。 （）
