水生态学杂志2016，Vol.37Issue(4)：84-88,5.DOI:10.15928/j.1674-3075.2016.04.013
金沙鲈鲤人工繁殖技术研究
Artificial Propagation of Percocypris pingi
摘要
Abstract
Percocypris pingi is a unique and precious wild fish endemic primarily to the upper Yangtze River and its tributaries in Sichuan,Guizhou and Yunnan Provinces.Due to environmental deterioration,construction of hydroe-lectric projects,pollution and overfishing,P.pingi populations have decreased sharply and disappeared from some of its original habitat.P.Pingi has been listed as a vulnerable species,the need to conserve this species is urgent and we describe here the artificial propagation of P.Pingi.Wild parental P.Pingi were collected from Zongxi Riv-er and Qixingguan River (Liuchong river basin)and raised to sexual maturity by artificial feeding.In July,201 4, wild P.pingi were induced to spawn by injecting mixed LRH,PG and HCG under the pectoral fin three times.The second injecting was 1 0 -1 5 days after the first injection and the time interval was just 45 -50 h between the sec-ond and the third injection.Results show that wild P.pingi can grow and develop in a pond culture with running water.After 45 -50 h,a total of 1 69 000 eggs were obtained (oxytocin rate,62% -67%).Artificial fertilization was carried out using the dry method (fertilization rate,79% -82%).The fertilized eggs were cultured under run-ning water in an incubator(0.4 m ×0.39 m ×0.1 5 m)at a water depth of 30 -35 cm and fertilized egg density of 1 0 000 -20 000 eggs per incubator.After 1 60 -1 82 h,the newly hatched P.pingi larvae were released with an average hatch time of 1 70.5 h (hatch rate,59% -61 %)and an average length of 7.8 -1 0.8 mm.The embryonic development of P.Pingi can be divided into 8 stages:fertilized egg,blastoderm,cleavage,blastula,gastrula, neurula,organ formation and larvae release from membrane.From minced red worm to artificial feed,the survival rate was 71 % -74% after 30d and the average body length of the fry was 1 .3 -2.4 cm.The successful artificial propagation of P.pingi provides a basis for culture and release of P.pingi.关键词
金沙鲈鲤/人工繁殖/技术研究Key words
Percocypris pingi/artificial propagation分类
农业科技引用本文复制引用
詹会祥,杨德国,李正友,王艳艳,周礼敬,吴兴兵,杨林..金沙鲈鲤人工繁殖技术研究[J].水生态学杂志,2016,37(4):84-88,5.基金项目
贵州省农委育种专项“鲈鲤种质资源保护及繁育技术研究”黔农育专字［2011］018号；“贵州省特色水产产业技术体系”项目（GZCYTX2013-011）；省农科院成果培育与人才培养项目“鲈鲤驯养及繁育技术研究”黔农科院 CR 合字（2014）46号；贵州省农委育种专项“鲈鲤人工驯养繁殖关键技术研究”黔农育专字［2015］022号。 （）
