水生态学杂志2016，Vol.37Issue(4)：95-100,6.DOI:10.15928/j.1674-3075.2016.04.015
圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验
Isolation,Identification and Drug Susceptibility Test of Aeromonas veronii from Coreius guichenoti
摘要
Abstract
The early life resource of Coreius guichenoti is decreasing significantly year by year in its primary habi-tat,the middle Jinsha River,and this is arousing concern about the need for conservation.Fish disease is the pri-mary problem for domestication and propagation of Coreius guichenoti.In July,201 5,an unknown bacterial disease broke out in the domesticated Coreius guichenoti in Wuhan,resulting in the death of over ten fish in a short time. To identify the pathogen and explore prevention strategies,a dominant strain,labeled YT01 ,was isolated from the spleen and kidney of moribund Coreius guichenoti.Biochemical analysis,1 6S rDNA sequencing and an antibiotic susceptibility test were carried out and the data generated will support efforts to control and prevent disease in Coreius guichenoti.The isolated YT01 strain,grown on BHI solid media developed round gray-white colonies with smooth surfaces,neat,opaque and slightly raised.The 1 6Sr DNA sequence of YT01 strain was 1 399 bp long and 1 00% homologous to Aeromonas veronii.A phylogenic tree was constructed based on the 1 6S rDNA sequence of the YT01 strain and 1 6S rDNA sequences from another 24 Aeromonas strains obtained from the NCBI GenBank data-base.The sequence of the YT01 strain clustered into a clade with Aeromonas veronii (KT964297.1 ;KJ650080.1 ;KM362731 .1 ),with a confidence level of 99%.Combined with the morphological characteristics and physiological and biochemical characteristics of the colony,the YT01 strain was conclusively identified as Aeromonas veronii.An-tibiotic susceptibility of the YT01 strain was tested using 1 7 common antibiotics.Results show that the YT01 strain is highly sensitive to 7 antibiotics,including florfenicol,moderately sensitive to rifampin and strongly resistant to 9 antibiotics,including neomycin.关键词
圆口铜鱼/维氏气单胞菌/生化鉴定/16SrDNA序列/药敏试验Key words
Coreius guichenoti/Aeromonas veronii/biochemical identification/1 6S rDNA sequence/susceptibility test分类
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曹恒源,乔晔,陈小娟,潘磊,谢建军,董纯,安苗,王小明,杨少荣..圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验[J].水生态学杂志,2016,37(4):95-100,6.基金项目
圆口铜鱼人工繁殖技术研究（2）（JGJ／0242013）。 （）
