实用医学杂志2016，Vol.32Issue(16)：2677-2679,3.DOI:10.3969/j.issn.1006-5725.2016.16.023
应用环介导等温扩增技术对分离自呼吸系统感染铜绿假单胞菌的快速检测
Establishment and optimization of loop-mediated isothermal amplification for rapid detection of pseu-domonas aeruginosa
林群 1冯洁仪 1黄金华 1黄少敏 1吴智刚2
作者信息
- 1. 528135 佛山市顺德区乐从医院
- 2. 528000 佛山市第一人民医院检验科
- 折叠
摘要
Abstract
Objective To establish a Loop-mediated Isothermal Amplification (LAMP) for Pseudomonas aeruginosa rapid detection. Method 152 P. aeruginosa strains isolated from nasal swabs and 30 reference strains were applied. P. aeruginosa ATCC15442 was used to develop LAMP amplification and evaluate sensitivity and specificity. Results Sensitivity of LAMP was 103 times higher than PCR, with DNA amount as 132 fg. When LAMP was applied to 30 reference strains and 152 P. aeruginosa strains , the specification was 100% while iden-tification rate reached 94.7%. Conclusion The establishment LAMP showed a promising prospect in P. aerugi-nosa rapid detection.关键词
环介导等温扩增技术/荧光结果判定法/铜绿假单胞杆菌/快速检测Key words
LAMP/Visual detection/SYBR Green/Rapid detection/P. aeruginosa引用本文复制引用
林群,冯洁仪,黄金华,黄少敏,吴智刚..应用环介导等温扩增技术对分离自呼吸系统感染铜绿假单胞菌的快速检测[J].实用医学杂志,2016,32(16):2677-2679,3.
