现代医药卫生2016，Vol.32Issue(15)：2298-2300,2303,4.DOI:10.3969/j.issn.1009-5519.2016.15.008
实用化改良Feulgen染色法的研究
Study on practical improved Feulgen staining method
黄荣祥 1余岚岚 1聂琨 1唐剑 1何文波 1马亮1
作者信息
- 1. 厦门麦克奥迪医学检验所，福建厦门361006
- 折叠
摘要
Abstract
Objective To explore a set of strongly repetitive,dyeing stable,short time-consuming and large-scale popu-larization improved Feulgen staining method (TEM35) to provide the methodological guidance for clinical scientific research ap-plications. Methods Twenty-one specimens of liquid-based cervical smear from March 2014 to May 2014 in our institute were selected. Each cervical specimen was made for 2 sections after mixing and all the sections were divided into two groups ,21 sec-tions in each group. The two groups were respectively stained by using the TEM35 and classical Feulgen staining method (TEM25). The Motic DNA image analysis system was used to measure the diploid cell nuclear (IOD) and its coefficient of variation (CV)value,and the measurement results were analyzed statistically. Results The overall IOD average value measured by TEM25 was 114.554 and which measured by TEM35 was 112.933,IOD had better consistency(Kendall′sτ=0.692,P<0.01). The CV value by TEM25 was 8.166,while which by TEM35 was 7.732,CV had slightly better consistency(Kendall′sτ=0.568,P<0.01). Conclusion The results obtained by using the practical improved Feulgen staining method are highly consistent with those by us-ing the classical Feulgen staining ,moreover which has short time-consuming and good staining specificity.关键词
染色与标记/病理学/图像处理,计算机辅助/DNA/肿瘤Key words
Staining and labeling/Pathology/Image processing,computer-assisted/DNA/Neoplasms引用本文复制引用
黄荣祥,余岚岚,聂琨,唐剑,何文波,马亮..实用化改良Feulgen染色法的研究[J].现代医药卫生,2016,32(15):2298-2300,2303,4.
