亚热带植物科学2016，Vol.45Issue(2)：122-126,5.DOI:10.3969/j.issn.1009-7791.2016.02.005
西瓜果实AGPL1基因启动子的序列分析
Cloning and Sequence Analysis ofAGPL1Promoters in Watermelon Fruit Tissue
摘要
Abstract
To make use of plant fruit specific express foreign proteins, 1.2 kbAGPL1gene promoter was amplified in watermelon leaves using PCR, and bioinformatics analysis ofAGPL1 promoter sequences was conducted. The results showed by PlantCARE database and PLACE database that AGPL1 gene promoter had number of typical core elements such as TATA-Box, CAAT-Box, high level transcription cis-acting element 5UTR Py-rich stretch, photoresponse elements including ACE, ATCC-motif, Box 4, I-Box, Sp1, TCCC-motif, GAG-motif, MNF1, two gibberellin response elements GARE-motif and P-box, cis-acting element related to protein metabolic regulation O2-site, cis-acting element related to salicylic acid reaction TCA-element, induced anaerocabically cis-acting element ARE, element involved in defense and stress responsiveness TC-rich repeats, circadian rhythm controlling element circadian and so on, all of which lays the groundwork for such research on regulation of PG genes in the future.关键词
西瓜/AGPL1/启动子Key words
watermelon/AGPL1/promoter分类
农业科技引用本文复制引用
周丽英,卜璐璐,杨春雷,高婷,田甜,杨正安..西瓜果实AGPL1基因启动子的序列分析[J].亚热带植物科学,2016,45(2):122-126,5.基金项目
国家自然科学基金项目(31260481、31460516)；云南省自然科学基金项目(2011FB049) （31260481、31460516）
