中国康复理论与实践2016，Vol.22Issue(8)：869-875,7.DOI:10.3969/j.issn.1006-9771.2016.08.001
完全性骶上脊髓损伤后神经源性膀胱大鼠模型的建立及尿流动力学分析
Establishment of Rat Model for Neurogenic Bladder after Complete Suprasacral Spinal Cord Injury and Urody-namic Analysis
摘要
Abstract
Objective To establish the rat model for neurogenic bladder after complete suprasacral spinal cord injury, and analyze the urodynamics. Methods Forty adult Sprague-Dawley rats were randomly divided into control group (n=10) and experimental group (n=30). The spinal cord injury model on T10 was made with modified spinal cord transaction. The Crede's method was used to urinate assistantly af-ter modeling. The amounts of urine and successful rat model everyday were recorded. The urodynamics was measured in both groups, and the morphology was observed with HE staining. Results Four rats died in the experimental group. The amount of urine was the least and sta-ble on the 18th day, and 24 rats were successfully modeled on the 21st day. Compared with the control group, maximum bladder capacity de-creased (t=3.150, P=0.021), bladder base pressure, maximum bladder pressure and leak point pressure increased (t>3.125, P<0.01), and blad-der compliancy decreased (t=5.330, P=0.002) in the experimental group. Conclusion The complete suprasacral after spinal cord injury of neurogenic bladder in a rat model of manufacturing method is of high reliability, good repeatability, and low coefficient of variable character-istics for spastistic neurogenic bladder basic experimental studies.关键词
骶上脊髓损伤/神经源性膀胱/动物模型/尿流动力学/大鼠Key words
suprasacral spinal cord injury/neurogenic bladder/animal model/urodynamics/rats分类
医药卫生引用本文复制引用
许明,张泓,刘继生,张健,尹秀婷,张雨辰..完全性骶上脊髓损伤后神经源性膀胱大鼠模型的建立及尿流动力学分析[J].中国康复理论与实践,2016,22(8):869-875,7.基金项目
国家自然科学基金面上项目(No.81473753)。 （No.81473753）
