中国康复理论与实践2016，Vol.22Issue(8)：884-891,8.DOI:10.3969/j.issn.1006-9771.2016.08.003
周围神经电刺激对脊髓损伤大鼠轴突再生的影响
Effect of Peripheral Nerve Electrical Stimulation on Axon Regeneration after Spinal Cord Injury in Rats
王永杰 1洪毅 2陈学明 1张亚奎1
作者信息
- 1. 首都医科大学附属北京潞河医院骨科，北京市101149
- 2. 中国康复研究中心北京博爱医院，北京市100068
- 折叠
摘要
Abstract
Objective To explore the effect of peripheral nerve electrical stimulation on axon regeneration after spinal cord injury (SCI) in rats. Methods Nighty-two healthy Sprague-Dawley rats were randomly divided into blank control group (n=12), control group (n=40) and experimental group (n=40). All groups were suffered NYU impaction to prepare T8 SCI models, the control group and the experimental group implanted stimulating electrode on the sciatica nerve. The experimental group received electric intervention in addition. They were evaluated with BBB score one day, one week, two weeks, four weeks and eight weeks after modeling; and with motor evoked potentials (MEP) one week, two weeks, four weeks and eight weeks after modeling. Morphological changes and the expression of neurofilament pro-tein (NF)-200 and glial fibers acid protein (GFAP) were observed by HE staining and immunohistochemistry one week, two weeks, four weeks and eight weeks after modeling. Results There was no significant difference in BBB scores among three groups (P>0.05) in all the time points except eight weeks (P<0.05). There was no significant difference in the amplitudes and latencies among three groups one week after modeling (P>0.05), however, there was significant difference two weeks, four weeks and eight weeks after modeling (P<0.05). All the groups showed syringomyelia and glial scar formation one week, two weeks, four weeks and eight weeks after modeling. There was no sig-nificant difference in NF-200 axon count among three groups one week after modeling (P>0.05), but was different two weeks, four weeks and eight weeks after modeling (P<0.05). There was no significant difference in GFAP area count among three groups in all the time points (P>0.05). Conclusion Implantable peripheral nerve electrical stimulation can improve conduction function and motor function in rats with SCI. And it may promote axonal regeneration of the injured segments.关键词
脊髓损伤/电刺激/轴突再生/大鼠Key words
spinal cord injury/electrical stimulation/axonal regeneration/rats分类
医药卫生引用本文复制引用
王永杰,洪毅,陈学明,张亚奎..周围神经电刺激对脊髓损伤大鼠轴突再生的影响[J].中国康复理论与实践,2016,22(8):884-891,8.
