中国免疫学杂志2016，Vol.32Issue(8)：1145-1149,5.DOI:10.3969/j.issn.1000-484X.2016.08.013
淫羊藿和女贞子提取物对骨质疏松症大鼠性激素功能水平的影响
Effect on sex hormone functional of extracts of Herba Epimedii/Fructus Ligustri Lucidi in osteoporosis rats
摘要
Abstract
Objective:To explore the effect on sex hormone functional of extracts of Herba Epimedii /Fructus Ligustri Lucidi in osteoporosis rats.Methods:50 cases of 3 months Wistar rats were rando mly divided into normal group ,model group,extract low dose group(extract-L),extract middle dose group(extract-M),extract high dose group(extract-H),10 cases in each group,in addition to the normal group,other 4 groups were bulided OP model by filling and feeding retinoic acid for 15 d,then normal group and model group were filling physiological saline;extract-L,extract-M,extract-H were respectively feeding extracts of Herba Epimedii /Fructus Ligustri Lucidi at 50 mg/kg,100 mg/kg,200 mg/kg,both for 3 weeks.Measured the bone mineral density (BMD),observed the femoral pathology change of left femur with hematoxylin and eosin ( HE ) staining, measured trabecular bone tissue morphology related parameters ,analyzed the hormone receptor protein expression with immunohistochemistry .Results:The BMD of the whole femur ,femur head,and femur neck in normal group were all significantly higher than that of model group ,the BMD of the whole femur ,femur head, and femur neck in extract-M and extract-H group were all significantly higher than that of model group ,the BMD of the whole femur in extract-L group was significantly higher than that of model group ,the differences were statistically significant (P<0.05 or P<0.01).The bone trabecular number had significantly increased ,integrity,continuity improved significantly ,bone histomorphometry such as Tb.Ar, Tb.Th,and Tb.N in model group were decreased ,and Tb.Sp was increased,extract-M and extract-H group had remarkable effect on each index ,while extract-L group only had a remarkable effect on Tb .Ar and Tb.Th ( P<0.05 or P<0.01 ) , compared with model group.The expression of AR , ER in normal groups were significantly higher than that of model group , the expression of AR , ER in extract-H group was significantly higher than that of model group , the differences were statistically significant ( P<0.05 or P<0.01 ) . Conclusion:This study demonstrated that the combination with TFE and TIFL had potential protective effects on osteoporosis rats induced by retinoic acid .The combination with TFE and TIFL is able to increase BMD ,reverse pathological changes of bone tissue ,co-ordinate hormones of the hypothalamic-pituitary-gonadal axis ,and upregulate the expression of sex hormone receptors .关键词
淫羊藿-女贞子/维甲酸/骨质疏松症/骨密度/性激素受体Key words
Herba epimedii/fructus ligustri lucidi/Retinoic acid/Osteoporosis/Bone mineral density/Sex hormone receptors分类
医药卫生引用本文复制引用
李凝旭,涂艳,沈莹,覃艳琼,雷超,柳湘洁..淫羊藿和女贞子提取物对骨质疏松症大鼠性激素功能水平的影响[J].中国免疫学杂志,2016,32(8):1145-1149,5.基金项目
本文为华中科技大学自主创新基金（2013QN127）。 （）
