中国免疫学杂志2016，Vol.32Issue(8)：1171-1174,1178,5.DOI:10.3969/j.issn.1000-484X.2016.08.019
复合前列腺特异性抗原的单克隆抗体制备及化学发光法免疫定量检测试剂研究
Generation of monoclonal antibodies against complexed prostate specific antigen and development of an antibody-based chemiluminescence immune quantifica-tion assay
摘要
Abstract
Objective:To construct a chemiluminescense immune quantification assay based one paired mAbs against complexed prostate specific antigen ( c-PSA).Methods:Six week-old female BALB/c mice were immunized with the commercial c-PSA antigen.After serum titer reaching a platform stage ,the spleen was immunized and fused with mouse myeloma cell lines ( Sp2/0 ) .The hybridoma were screened by indirect ELISA ,and eight generated antibodies were paired to obtain a quantitative analysis of the chemical luminescence.Results:7D6 specifically recognized c-PSA,while 1A10 recognized total PSA(t-PSA).And the paired antibody 1A10/7D6 were determined to successfully construct a chemiluminescense immune response quantitative detection method through the detection of c-PSA standard and clinical serum samples .had,positive samples have statistically significant difference ( P<0.000 1 ) with negative samples.And the correlation coefficient R 2 was 0.97 between our c-PSA quantitative results and that of the Siemens c-PSA chemiluminescense immunoassay kit .The detection linear range was 0.1-100 ng/ml,and the sensitivity was 0.005 ng/ml.Conclusion:The paired monoclonal antibodies specifically detecting c-PSA were generated and a c-PSA chemiluminescense immunoassay were developed in this study .The detection capability of our method was comparable with that of the international commercial kit .关键词
复合前列腺特异性抗原/单克隆抗体/化学发光/定量试剂Key words
Complexed prostate specific antigen ( c-PSA)/Monoclonal antibody/Chemiluminescense/Quantification reagent分类
医药卫生引用本文复制引用
詹炉停,郑子峥,温桂平,赵敏,依含,刘江武,郭小怡,林海军,黄刘女,夏宁邵..复合前列腺特异性抗原的单克隆抗体制备及化学发光法免疫定量检测试剂研究[J].中国免疫学杂志,2016,32(8):1171-1174,1178,5.基金项目
本文为863计划资助项目（No．2011AA02A101；2012AA02A307）。 （）
