中国免疫学杂志2016，Vol.32Issue(8)：1175-1178,4.DOI:10.3969/j.issn.1000-484X.2016.08.020
磁微粒化学发光法与酶联免疫吸附法测定抗中性粒细胞胞浆抗体的结果比较
Comparison of chemiluminescent microparticle immunoassay and enzyme-linked immunosorbent assay for determination of anti-neutrophil cytoplasm antibodies
摘要
Abstract
Objective:To compare the performance of chemiluminescent microparticle immunoassay ( CMIA) and enzyme-linked immunosorbent assay ( ELISA) for the determination of Anti-PR3 and Anti-MPO.Methods:Concentration of Anti-PR3 and Anti-MPO in serum samples from 166 patients with autoimmune diseases and 50 healthy donors were determined by using CMIA (Method A) and ELISA(Method B),respectively.The results from both assays were analyzed and compared by statistical methods .Results:Method A showed better intra-assay reproducibility and inter-assay reproducibility than Method B for the determination of high ,medium and low levels of control serum .Both methods met the accuracy requirement .The correlation coefficient of Anti-PR3 and Anti-MPO were 0.987 8 and 0.989 6 for Method A and Method B ,respectively.And the Kappa coefficients were 0.897 and 0.882 for Method A and Method B,respectively.Conclusion:The performance of Method A is superior to Method B for the deter-mination of Anti-PR3 and Anti-MPO, which makes Method A to be a potentially better choice for clinical application .关键词
抗中性粒细胞胞浆抗体/髓过氧化物酶/蛋白酶3/酶联免疫吸附法/磁微粒化学发光法Key words
Anti-neutrophil cytoplasm antibodies (ANCA)/Myeloperoxidase(MPO)/proteinase 3(PR3)/Enzyme-Linked Im-munoSorbent Assay/Chemiluminescent Microparticle ImmunoAssay分类
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张立娟..磁微粒化学发光法与酶联免疫吸附法测定抗中性粒细胞胞浆抗体的结果比较[J].中国免疫学杂志,2016,32(8):1175-1178,4.
