中国医学创新2016，Vol.13Issue(21)：18-20,21,4.DOI:10.3969/j.issn.1674-4985.2016.21.005
人脑胶质瘤干细胞增殖中NOTCH信号的调控作用
NOTCH Signal Regulating Role in Human Brain Glioma Stem Cells Proliferation
摘要
Abstract
Objective:To study the NOTCH signal regulating role in human brain glioma stem cell proliferation.Method:Using magnetic beads immune method extracted from the organization for human brain glioma and glioma cell line,separated the brain glioma stem cells,cells for in vitro culture,used the fluorescent quantitative PCR and immunohistochemical method to detect the related indicators,observed the NOTCH signal pathway in the brain glioma stem cells proliferation regulation function.Result:Patients with normal brain tissue protein accumulation NOTCH-1 optical density value was (294.9±29.5),low and highly malignant gliomas NOTCH-1 protein accumulation optical density value was respectively (5046.0±120.9) and (11 026.2±169.1). Low and highly malignant gliomas NOTCH-1 protein expression levels significantly higher than patients with normal brain tissue,and highly malignant gliomas NOTCH-1 protein expression levels was significantly increased than low grade gliomas(P<0.05).In CD133+glioma cells,NOTCH-1,CBF-1 and HES-1 gene expression level of CD133 glioma cells increased significantly(P<0.05).Conclusion:The NOTCH-1 gene is the high level expression in human brain gliomas.In the process of human glioma stem cells proliferation,NOTCH,CBF-1 and HES-1 key genes has high expression,such as NOTCH signaling pathways in the brain glioma stem cells proliferation plays an important regulatory role in the process,it is worth of further study.关键词
人脑胶质瘤干细胞/增殖/NOTCH信号/调控作用Key words
Human brain glioma stem cells/Proliferation/NOTCH signal/Regulation effect引用本文复制引用
徐菲,陈东,胡继良..人脑胶质瘤干细胞增殖中NOTCH信号的调控作用[J].中国医学创新,2016,13(21):18-20,21,4.基金项目
深圳市知识创新计划-基础研究项目 （）
