中国医学创新2016，Vol.13Issue(22)：24-27,28,5.DOI:10.3969/j.issn.1674-4985.2016.22.006
雷公藤甲素体外对人表皮干细胞增殖的调控作用
In Vitro Regulatory Effect of Triptolide on Proliferation of Human Epidermal Stem Cells
摘要
Abstract
Objective:To investigate the effect of Triptolide on the proliferation of human epidermal stem cells(hESCs)in vitro.Method:hESCs was isolated from human foreskin by the method of protease-collagenⅣ-coated adhesion.The expression of integrinsβ1,keratin 19 and keratin 10 on the generation of hESCs were examined by immunocytochemical method.After the generation of hESCs were cultured in epilife medium containing Triptolide of different concentrations respectively.The shape,clone formation and proliferation of the cell were observed through inverted microscope.The effect of Triptolide was evaluate by MTT method on proliferation of human epidermal stem cells in vitro.Result:The results of immunocytochemistry showed keratin 19 and intergrin β1 were positively expressed at the generation of hESCs,but keratin 10 was negative.MTT assay showed that the proliferation of human epidermal stem cells was significantly inhibited, and there was a concentration dependent.Conclusion:Triptolide can effectively inhibit the proliferation of human epidermal stem cells in vitro.关键词
雷公藤甲素/表皮干细胞/增殖/角蛋白19/β1整合素Key words
Triptolide/Epidermal stem cells/Proliferation/Keratin 19/Integrinsβ1引用本文复制引用
江惟苏,何友珍,王鹏,欧阳资章,汤素环..雷公藤甲素体外对人表皮干细胞增殖的调控作用[J].中国医学创新,2016,13(22):24-27,28,5.基金项目
清远市科技计划项目 （）
