中国医学创新2016，Vol.13Issue(23)：13-16,4.DOI:10.3969/j.issn.1674-4985.2016.23.004
腺嘌呤、鸟嘌呤致高尿酸血症大鼠肝脏损害的研究
Studies of Hepatic Lesion on Hyperuricemia Rat-model Induced by Adenine and Guanine
摘要
Abstract
Objective:To observe adenine and guanine effect liver function of hyperuricemia-model rat, and the changes under ultra microstructure.Method:The 36 male Wistar rats were randomly divided into 6 groups (A-control group,B-model group,C-adenine group,D-adenine starch paste group,E-adenine and guanine group,F-guanine group),each group of 6 cases.Groups B, C, D, E, F continuously given Yeast Extract Solution 15 g/(kg·d) to fill the stomach,7 days induced hyperuricemia-model rats.After the success of the building, group B for starch paste to fill the stomach.Group C:20 mg/(kg·d) adenine starch paste mixture.Group D:10 mg/(kg·d) adenine starch paste mixture.Group E:10 mg/(kg·d) adenine mixed 10 mg/(kg·d) guanine starch suspension.Group F:20 mg/(kg·d) guanine starch paste mixture to fill the stomach.For 14 days,the determination serum ALT,AST,UA and liver tissue electron microscope observation of liver injury of each group rats.Result:(1)Ultra microstructure observation the lysosome of group C increased obviously,scattered around the nucleus,and had dark grain material.Group D lysosome amount increased slightly,increased lipid drops, enter the lysosome.Group E the amount of lysosome increasing, increased lipid droplets, appear dark granular material.Group F bile duct in a small dark granular material.(2)ALT,AST of C-F groups were compared with group B,the differences were statistically significant(P<0.05),UA of C-E groups were compared with group B, the differences were statistically significant(P<0.05).And ALT,AST,UA levels of group C,D,E were higher than group F.Conclusion:Adenine and guanine both lead to liver damage of hyperuricemia-model rats. Adenine <br> cause ALT and AST levels significantly in hyperuricemia-model rats, the effect is higher than the same dose of guanine.Adenine has obvious effect to rat blood uric acid levels.关键词
腺嘌呤/高尿酸血症/鸟嘌呤/肝脏损害/超微结构Key words
Adenine/Hyperuricemia/Guanine/Liver damage/Ultra microstructure引用本文复制引用
宋燕郡,于维森,殷凡,宋扬..腺嘌呤、鸟嘌呤致高尿酸血症大鼠肝脏损害的研究[J].中国医学创新,2016,13(23):13-16,4.基金项目
青岛市卫计委立项项目 （）
