中国医学创新2016，Vol.13Issue(23)：17-19,20,4.DOI:10.3969/j.issn.1674-4985.2016.23.005
LOX-1受体启动子基因调控及其抗大鼠肾脏损害的作用
The Effects of LOX-1 Receptor Gene Promoter Regulation on the Rat Renal Injury
摘要
Abstract
Objective:To explore the regulative effects of pyrrole-imidazole polyamide (PIP) on the lectin-like oxidized Low-density lipoprotein receptor-1(LOX-1)receptor gene promoter,and to analyze the effects of PIP on the rat renal injury.Method:The 50 male Sprague Dawley rats were randomly divided into five groups with 10 rats in each group:group A,normal diet,no PIP;group B,high-fat diet,no PIP;group C1, high-fat diet,PIP 10 μg/(kg·d)i.v.;group C2, high-fat diet,PIP 5 μg /(kg·d)i.v.;group D,high-fat diet,rosuvastatin 5 mg/(kg·d)intragastric administration.At the 8th week,the mRNA expressions of LOX-1 and MMP-9 in renal tissue were detected by real-time PCR. The levels of serum cholesterol (TC),triglycefide (TG),HDL-C and LDL-C were measured.The 24 hour urine protein excretion,serum BUN and Scr were measured.Result:Compared with group B,the mRNA expressions of LOX-1 and MMP-9 were significantly decreased in the rat kidney of group C1 and C2(P<0.01).Compared with group B,the serum TC,TG,HDL-C and LDL-C had no statistical differences in group C1 or C2(P>0.05).Compared with group B,the levels of 24 hour urine protein excretion were significantly decreased in the rats of group C1 and C2(P<0.01),but the serum BUN and Scr had no statistical differences compared with group A(P>0.05).Conclusion:The expression of LOX-1 mRNA was significantly decreased by the PIP in the kidney of high-fat diet rats,which may be involved in the inhibition of the renal injury.关键词
凝集素样氧化型低密度脂蛋白受体-1/吡咯-咪唑聚酰胺/肾脏损害Key words
Lectin-like oxidized Low-density lipoprotein receptor-1/Polyamide/Renal injury引用本文复制引用
姚恩辉,黄津华,周振智,方军,石黎明,叶洪..LOX-1受体启动子基因调控及其抗大鼠肾脏损害的作用[J].中国医学创新,2016,13(23):17-19,20,4.基金项目
福建省医学创新课题资助 （）
