安徽农业科学2016，Vol.44Issue(20)：4-7,4.
高效液相色谱法测定天女木兰叶中游离酚和结合酚
Determination of Free Phenol and Combined Phenol in Leaves of Magnolia sieboldii K.Koch by High Performance Liquid Chroma-tography
摘要
Abstract
Objective] To further understand the type and content of phenol in Magnolia sieboldii K.Koch.[ Method] High performance liq-uid chromatography ( HPLC) was used to qualitatively and quantitatively measure the free phenol, combined phenol and ester phenol in leav-es.[Result] Among the four detected phenol, M.sieboldi i leaves contained two free phenols (coumaric acid and chlorogenic acid), four combined phenols ( caffeic acid, coumaric acid and catechin,coumarin ) and two ester phenols ( coumarin and coumaric acid) .Various forms of coumaric acid widely existed in leaves.[ Conclusion] Detecting free phenol and combined phenol in M.sieob ldii leaves by HPLC is accu-rate and reliable.关键词
高效液相色谱/天女木兰/游离酚/结合酚/酯化酚Key words
HPLC/Magnolia sieboldii K.Koch/Free phenol/Combined phenol/Ester phenol分类
农业科技引用本文复制引用
高红兵,杜风国,王欢..高效液相色谱法测定天女木兰叶中游离酚和结合酚[J].安徽农业科学,2016,44(20):4-7,4.基金项目
吉林省科技厅项目（20140101016JC）；吉林省教育厅项目（吉教合字2014第172号）。 （）
