安徽农业科学2016，Vol.44Issue(20)：63-65,3.
响应面法优化费菜SOD提取条件的研究
Study on Optimizing Extracting Conditions of SOD in Sedum aizoon by the Response Surface Method
王美 1武纪汝 1刘艳芳 1王文斗1
作者信息
- 1. 山西师范大学生命科学学院，山西临汾041000
- 折叠
摘要
Abstract
Objective] The aim was to optimize extracting conditions of SOD in Sedum aizoon by response surface method.[Method] First, the single factor optimization tests were carried out.Three single factors are time, pH and solid-to-liquid ratio respectively.By Design expert 8.0.5 software, 17 kinds of test scheme were designed.The protein yield of each scheme was obtained, Design expert 8.0.5 software was used to con-duct variance analysis, interactive analysis among factors, corresponding mathematical model was obtained.[Result] The results showed that in single factor experiments, the optimal time is 1 hour, pH is 7.8, and solid-to-liquid ratio is 1∶15 g/mL.Factors interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon is time 0.98 h, pH 7.79, solid-to-liquid ratio 1∶13.71 g/mL.By the method of py-rogallol autoxidation, the extracted SOD enzyme activity was measured, that is 12 U/g.[Conclusion] The results can provide reference for fur-ther utilization and research of Sedum aizoon resources.关键词
费菜/SOD/响应面法Key words
Sedum aizoon/FSOD/Response surface method分类
农业科技引用本文复制引用
王美,武纪汝,刘艳芳,王文斗..响应面法优化费菜SOD提取条件的研究[J].安徽农业科学,2016,44(20):63-65,3.
