北京中医药大学学报2016，Vol.39Issue(8)：653-658,6.DOI:10.3969/j.issn.1006-2157.2016.08.009
不同证候舌苔 HSP70、IL-8 mRNA 表达与胃黏膜 HP 感染和病理改变的关联
Correlation of mRNA expressions of HSP70 and IL-8 in coating on tongue,HP infection and pathological changes of gastric mucosa in pa-tients with different TCM patterns
摘要
Abstract
Objective Taking patients with Helicobacter pylori (HP)-related gatrosis as the subjects,so as to study correlation of TCMpatterns,HP infection,pathological changes of gastric mucosa and mRNA expression of heat shock protein70 (HSP 70)and interleukin-8 (IL-8).Methods In the First Affilia-ted Hospital of Guangzhou University of Chinese Medicine,total 96 patients with HP-related gastrosis were included from March to November in 2010,including 72 cases of chronic gastritis and 24 cases of peptic ulcer,with 68 cases of splenogastric hygropyrexia pattern(SGHP)and 28 cases of spleen deficien-cy pattern (SDP),and 14 healthy volunteers recruited were assigned into control group.Using rapid ure-ase test,methylene blue test and HE staining to detect HP infection and gastric mucosa pathological changes;using fluorescence quantitative PCR to detect mRNA expression of HSP70 and IL-8 of tongue coating.Results HP infection rate in SGHP was higher than that in SDP,while more severe and more active than the latter;HP infection in patients was more severe and active than that of subjects without HP infection with the same TCM pattern(P <0.01 ).The gastric mucosal lymphoid follicles,atrophy with intestinal metaplasia and /or hyperplasia pathological changes due to HP infection were significantly higher than no-infected subjects in SGHP and SDP.HSP70,IL-8 mRNA expressions of patients were higher than no-infected ones in tongue coating.More severe HP infection was,more IL-8 mRNA ex-pressed.HSP70 mRNA expression in SGHP was lower than SDP,but IL-8 expression was higher.HSP70 expression of HP infected patients with SDP was significantly higher than no-infected people with SGHP and SDP (P <0.05).Conclusion The correlation of HSP70,IL-8 mRNA expression in tongue coat-ing,HP infected gastric mucosa and atrophy with intestinal metaplasia and /or hyperplasia pathological changes was proved,which showed specific features with TCMpatterns.The results would provide refer-ence to pattern differentiation for HP-related gastrosis.关键词
HP 相关胃病/证候/舌苔/热休克蛋白 70/白细胞介素-8Key words
Helicobacter pylori-related gastrosis/TCM pattern/tongue coating/heat shock protein 70/interleukin-8分类
医药卫生引用本文复制引用
胡玲,程明,李培武,罗琦,陈苇菁,劳绍贤..不同证候舌苔 HSP70、IL-8 mRNA 表达与胃黏膜 HP 感染和病理改变的关联[J].北京中医药大学学报,2016,39(8):653-658,6.基金项目
国家自然科学基金项目（No．30772689，81373563），中央财政支持地方高校发展专项（No．财教［2013］338号），广东省教育厅华南中医药协同创新中心项目 （）
