北京中医药大学学报2016，Vol.39Issue(8)：679-684,6.DOI:10.3969/j.issn.1006-2157.2016.08.013
羟基红花黄色素A抑制异常增殖血管内皮细胞的机制研究
Hydroxysafflor yellow A inhibited abnormal proliferation of vascular en-dothelial cells
摘要
Abstract
Objective To study the inhibitory mechanism of hydroxysafflor yellow A (HSYA)on abnor-mal proliferation of vascular endothelial cells.Methods Co-cultured model in vitro was established, with supernatant fraction of LS180 human colon adenocarcinoma cells tumor cells and ECV304 human umbilical vein endothelial cells.Then mRNA expressions of vascular endothelial growth factors (VEGF) and VEGF receptors (KDR),basic fibroblast growth factor (bFGF)and bFGF receptors(bFGFR),pro-teoglycan related to cell proliferation (HSPG2),p53 and c-myc were detected using real-time fluores-cence quantitative PCR;protein expressions of VEFG,KDR,bFGF and bFGFR in supernatant fraction were measured by ELISA.Results In the cell co-cultured model,the HSYA concentration of 0.66 and 0.33 mg/L inhibited mRNA and protein expressions of VEGF,KDR,bFGF and bFGFR ,inhibited mR-NA expressions of c-myc and HSPG2,while upregulated p53 mRNA expression.Conclusion HSYA in-hibited angiogenesis and abnormal proliferation of vascular endothelial cells in co-cultured model by regu-lating expressions of VEGF,VEGFR,bFGF,bFGFR,HSPG2,c-myc,wild type p53.HSYA may be a potential tumor angiogenesis inhibitor.关键词
羟基红花黄色素 A/血管内皮细胞/血管内皮生长因子/肿瘤/人结肠腺癌细胞Key words
hydroxysafflor yellow A/vascular endothelial cell/vascular endothelial growth factor (VEGF)/tumor/LS180 human colorectal adenocarcinoma cells分类
医药卫生引用本文复制引用
王熙熙,臧宝霞,张前,王景景,王旭,范盎然,于雪,胡京红,解华,徐莹莹,刘丽..羟基红花黄色素A抑制异常增殖血管内皮细胞的机制研究[J].北京中医药大学学报,2016,39(8):679-684,6.基金项目
国家自然科学基金项目（No．30572436），北京中医药大学研究生自主课题 （）
