草业学报2016，Vol.25Issue(8)：145-153,9.DOI:10.11686/cyxb2015497
青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究
Screening of rhizobia of common vetch (Vicia sativa)in Qinghai,and assessment of symbiont salt tolerance
摘要
Abstract
Common Vetch (Vicia sativa )is a traditional legume crop in China which is widely used as a green manure and for fodder.However,there has been little study to date of the rhizobia of common vetch.This study aimed to identify rhizobial strains of common vetch with superior symbiotic properties,and also greater salt tolerance.Root nodule samples were collected from the major production areas of common vetch in Qing-hai,and 61 rhizobial strains were isolated and identified.Using a hydroponics inoculation technique and princi-pal component analysis,5 strains with stronger ability to promote nodulation and growth of the host plants were identified,and designated J1-3-2,J2-6-2,J3-12-1,J4-3-3 and J5-5-3.The salt tolerance of these 5 strains was examined by single plant culture.Strain J5-5-3 was tolerant of NaCl concentrations up to about 0.9%, while the other 4 strains could tolerate up to 1.2% NaCl concentration.Three distinct levels of salt tolerance were evident:J3-12-1 and J2-6-2 displayed the strongest tolerance,J1-3-2 and J4-3-3 displayed medium toler-ance,and J5-5-3 the weakest tolerance.One strain from each of these groups was then evaluated in a pot exper-iment to asses nodulation of the host under differing salinity levels of 0.15% or 0.3% NaCl.When exposed to 0.15% NaCl concentration,the largest number of root nodules was observed in plants inoculated with J5-5-3, and no significant differences among the three rhzobial strains were found when exposed to 0.30% NaCl con-centration.J3-12-1 and J5-5-3 inoculation resulted in increased dry weight (P <0.05)of the host plants under NaCl concentrations of 0.15% and 0.30%.Inoculation with strain J5-5-3 increased plant nitrogen content (P <0.05)under the two levels of NaCl stress and increased nitrogen uptake (P <0.05)under the 0.15% NaCl stress,while the strain J3-12-1 significantly increased the nitrogen uptake (P <0.05)of the host plants under 0.15% NaCl stress.It was concluded that the strains J3-12-1 and J5-5-3 are suitable as inoculant rhizobial strains to increase the biomass and nitrogen content of common vetch under salt stress.关键词
箭筈豌豆/根瘤菌/盐胁迫Key words
common vetch/rhizobia/salt stress引用本文复制引用
王雪翠,王艳秋,马晓彤,韩梅,曹卫东,张宏亮,白金顺,曾闹华,高嵩涓,周国朋..青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究[J].草业学报,2016,25(8):145-153,9.基金项目
公益性行业（农业）科研专项（201103005），中国农业科学院科技创新工程，国家农作物种质资源平台运行服务项目（NICGR2015-019），农作物种质资源保护与利用专项（2015NWB042）和青海省“昆仑学者”计划资助。 （）
