南方医科大学学报2016，Vol.36Issue(8)：1034-1040,7.DOI:10.3969/j.issn.1673-4254.2016.08.02
艾塞那肽通过SDF-1/CXCR-4/Rho GTPase通路增强脂肪来源间充质干细胞的趋化性迁移
Exenatide promotes chemotactic migration of adipose-derived stem cells through SDF-1/CX-CR-4/Rho GTPase pathway
摘要
Abstract
Objective To investigate the effect of exenatide on chemotactic migration of adipose-derived stem cells (ADSCs) and confirm that Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 migration pathway. Methods ADSCs were isolated, cultured, identified by flow cytometry, and induced to differentiate in vitro. RTCA xCELLigence system was used to analyze the effect of exenatide on ADSC proliferation. The effects of exenatide at different concentrations, AMD3100 (CXCR-4 antagonist), and CCG-1423 (Rho GTPase antagonist) on chemotactic migration of ADSCs were tested using Transwell assay. The expression of CXCR-4 in exenatide-treated ADSCs was measured by flow cytometry and Western blotting. Active Rho pull-down detection kit was used to detect the expression of Rho GTPase. Laser confocal microscopy was used to observe the formation of stress fibers in ADSCs with different treatments. Result Exenatide treatment for 24 h had no significant effect on ADSC proliferation. Exenatide obviously promoted chemotactic migration of ADSCs in a concentration-dependent manner, and this effect was blocked by either AMD3100 or CCG-1423. Both flow cytometry and Western blotting showed that exenatide dose-dependently up-regulated CXCR-4 expression in ADSCs. Western blotting showed that the expression of Rho GTPase was related to SDF-1/CXCR-4 pathway, and laser confocal microscopy revealed that the formation of stress fibers in ADSCs was related to SDF-1/CXCR-4/ Rho GTPase pathway. Conclusion Exenatide promotes chemotactic migration of ADSCs, and Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 pathway.关键词
脂肪间充质干细胞/艾塞那肽/趋化性迁移/SDF-1/CXCR-4通路/Rho GTPaseKey words
adipose-derived stem cells/exenatide/chemotaxis migration/SDF-1/CXCR-4 pathway/Rho GTPase引用本文复制引用
马强,杨俊杰,周浩,张颖,陈韵岱..艾塞那肽通过SDF-1/CXCR-4/Rho GTPase通路增强脂肪来源间充质干细胞的趋化性迁移[J].南方医科大学学报,2016,36(8):1034-1040,7.基金项目
国家自然科学基金（81400229）Supported by National Natural Science Foundation of China (81400229) （81400229）
