南方医科大学学报2016，Vol.36Issue(8)：1094-1099,6.DOI:10.3969/j.issn.1673-4254.2016.08.12
大鼠创伤性颅脑损伤后脑室下区内源性神经干细胞的增殖与分化
Proliferation and differentiation of endogenous neural stem cells in subventricular zone in rats after traumatic craniocerebral injury
摘要
Abstract
Objective To observe the time course of proliferation and differentiation of neural stem cells (NSCs) in the subventricular zone (SVZ) of rats following traumatic craniocerebral injury (TBI). Methods Forty-eight SD rats were randomized into 3 groups, namely the control group without any treatment, the sham-operated group with scalp incision and preparation of a cranial window, and TBI group with craniocerebral injury induced by Feeney's method. With nestin and BrdU as two cell markers, NSE as the neuron-specific marker and GFAP as the glial cell marker, immunofluorescence assay with double labeled antibodies was performed to examine the proliferation and differentiation of endogenous NSCs in the SVZ at different time points after TBI. Results The numbers of cells positive for nestin/NSE, nestin/GFAP, BrdU/NSE, and BrdU/GFAP in the SVZ of the rats increased significantly after TBI. The positive cells began to increase at 1 day after TBI, reached the peak level at day 3 and became normal at day 14, showing significant differences between the time points of measurement following TBI and from the cell numbers in the control group measured at the same time points. The cells positive for nestin/GFAP showed the most distinct increase in the SVZ of the rats with TBI. Conclusion TBI results in mobilization of the NSCs in the SVZ on the injured side to cause the proliferation and differentiation of the endogenous NSCs. The SVZ is one of the most important germinal centers of NSC proliferation and differentiation.关键词
颅脑损伤/脑室下区/神经干细胞/增殖分化Key words
craniocerebral injury/subventricular zone/neural stem cells/proliferation/differentiation引用本文复制引用
隋立森,余佳彬,姜晓丹..大鼠创伤性颅脑损伤后脑室下区内源性神经干细胞的增殖与分化[J].南方医科大学学报,2016,36(8):1094-1099,6.基金项目
国家自然科学基金（81171179）；广东省自筹经费类科技厅科技项目（粤科规划字［2013］137号）Supported by National Natural Science Foundation of China (81171179) （81171179）
