南方医科大学学报2016，Vol.36Issue(8)：1110-1116,7.DOI:10.3969/j.issn.1673-4254.2016.08.15
MiR-124通过靶向PRRX1调节结直肠癌细胞的辐射敏感性
miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1
林水苗 1夏琼 2张余琴 3孙爱民 1石玉生 1郑林 4陈龙华5
作者信息
- 1. 南方医科大学 南方医院放疗科，广东 广州 510515
- 2. 南方医科大学 影像系，广东 广州 510515
- 3. 暨南大学附属第一医院肿瘤科，广东 广州 510632
- 4. 南方医科大学 基础医学院病理教研室，广东 广州 510515
- 5. 南方医科大学 南方医院病理科，广东 广州 510515
- 折叠
摘要
Abstract
Objective To detect the expression of miR-124 in colorectal carcinoma (CRC) cells and tissue specimens and analyze its association with the radiosensitivity of the cells. Methods The expression of miR-124 in CRC cell lines and tissues were detected using qRT-PCR. The effect of miR-124 in modulating cell radiosensitivity was assessed in CRC cells with miRNA-124 overexpression and miRNA- 124 knockdown, and bioinformatics prediction and dual luciferase reporter system were employed to identify the direct target of miR-124. Results miR-124 expression was down-regulated in CRC cell lines and tissues. CRC cells over-expressing miR-124 showed an obviously enhanced radiosensitivity, whereas miR-124 knockdown resulted in a reduced radiosensitivity of the cells. Bioinformatics prediction and dual luciferase reporter system verified PRRX1 as a direct target of miR-124, which regulated the radiosensitivity of CRC cells by directly inhibiting PRRX1. Conclusion miR-124 can enhance the radiosensitivity of CRC cells by directly targeting PRRX1, which provides a target for improving the therapeutic effect of radiotherapy of CRC.关键词
结直肠癌/miR-124/放射敏感性/PRRX1Key words
colorectalcancer/miR-124/radiosensitivity/PRRX1引用本文复制引用
林水苗,夏琼,张余琴,孙爱民,石玉生,郑林,陈龙华..MiR-124通过靶向PRRX1调节结直肠癌细胞的辐射敏感性[J].南方医科大学学报,2016,36(8):1110-1116,7.
