南方医科大学学报2016，Vol.36Issue(8)：1148-1152,5.DOI:10.3969/j.issn.1673-4254.2016.08.22
上皮钠离子通道对大鼠破骨细胞分化和骨吸收功能的影响
Role of epithelial sodium channel in rat osteoclast differentiation and bone resorption
摘要
Abstract
Objective To explore the role of epithelial sodium channel (ENaC) in regulating the functional activity of osteoclasts. Methods Multinucleated osteoclasts were obtained by inducing the differentiation of rat bone marrow cells with macrophage colony-stimulating factor (M-CSF) and RANKL. The osteoclasts were exposed to different concentrations of the ENaC inhibitor amiloride, and the expression of ENaC on osteoclasts was examined using immunofluorescence technique. The osteoclasts were identified with tartrate-resistant acid phosphatase (TRAP) staining, and the positive cells were incubated with fresh bovine femoral bone slices and the number of bone absorption pits was counted by computer-aided image processing. RT-PCR was performed to analyze the expression of cathepsin K in the osteoclasts. Results Exposure to different concentrations of amiloride significantly inhibited the expression of ENaC and reduced the number of TRAP-positive osteoclasts. Exposure of the osteoclasts to amiloride also reduced the number of bone resorption pits on bone slices and the expression of osteoclast-specific gene cathepsin K. Conclusions ENaC may participate in the regulation of osteoclast differentiation and bone resorption, suggesting its role in functional regulation of the osteoclasts and a possibly new signaling pathway related with ENaC regulation for modulating bone metabolism.关键词
破骨细胞/上皮钠离子通道/阿米洛利/免疫荧光Key words
osteoclast cell/epithelial sodium channel/amiloride/immunofluorescence引用本文复制引用
虎松艳,李青南,金小冬,张豪,陈珺,杨国柱,王晓东,唐璐,陆幸妍,卢丽..上皮钠离子通道对大鼠破骨细胞分化和骨吸收功能的影响[J].南方医科大学学报,2016,36(8):1148-1152,5.基金项目
广东省科技计划项目 （）
