工业微生物2016,Vol.46Issue(4):8-13,6.DOI:10.3969/j.issn.1001-6678.2016.04.002
盾壳霉抗逆性菌株的筛选
Breeding of stress resistance mutants about bio-control fungi Coniothyrium minitans
摘要
Abstract
Tolerance of wild-type Coniothyrium minitans strain JN-CM were determined to selected pesticides and fertilizer. The results indicated that glyphosate, imidacloprid and phosphate fertilizer had significant inhibitory effects on the strain. Then the mutant stain with a certain degree of tolerance of glyphosate, imidacloprid and phosphate fertilizer were successfully screened through ultraviolet mutagenesis and named as glyphosate ( UV-G) , imidacloprid ( UV-I) and phosphate fertilizer ( UV-P ) , respectively. Three mutant strains had good genetic stability. Mutants had no significant differences between ability of growth and infection-associated enzyme activity if compared with those of wild-type strain, which showed their good application prospects.关键词
盾壳霉/突变菌株/紫外诱变Key words
Coniothyrium minitans/mutant strain/ultraviolet mutagenesis引用本文复制引用
金强,段作营,夏森玉,沈志松,李华钟,金坚..盾壳霉抗逆性菌株的筛选[J].工业微生物,2016,46(4):8-13,6.基金项目
2015江苏省重点研发计划(现代农业)重点项目(BE2015309)。 (现代农业)