工业微生物2016，Vol.46Issue(4)：14-18,5.DOI:10.3969/j.issn.1001-6678.2016.04.003
乳糖诱导剂促进P450 BM-3在大肠杆菌中可溶性表达的研究
Enhanced soluble expression of cytochrome P450 BM-3 in E. coli by using lactose as inducer
张彭湃 1王松廷1
作者信息
- 1. 河南大学生命科学学院，河南 开封475004
- 折叠
摘要
Abstract
P450 BM-3 is a monooxygenase with the potential for industrial application. It can catalyze saturated fatty acids hydroxylation. In order to enhance the expression level and the solubility of P450 BM-3 in Escherichia coli BL21 (DE3), studies was carried out with lactose as the inducer. The expressions conditions in shake flask were optimized. It was found that while 2. 0 g/L lactose was added into the culture (OD600=0. 7~1. 5) and incubated at 30℃ for 10 hours, the yield of P450 BM-3 arrived at the maximum (80 mg/L). With lactose induction, the biomass was doubled, the amount of P450 BM-3 achieved 626. 8 nmol/L, which was 3. 13 times of that with IPTG induction. The solubility of P450 BM3 was also improved, for the inclusion bodies decreased to 10%. These data indicated that lactose was effective to improve the expression of P450 BM-3 in E. coli and would be conducive to the soluble expression of P450 BM-3.关键词
P450 BM-3/乳糖/IPTG/诱导剂/可溶性表达Key words
P450 BM-3/lactose/IPTG/inducer/soluble expression引用本文复制引用
张彭湃,王松廷..乳糖诱导剂促进P450 BM-3在大肠杆菌中可溶性表达的研究[J].工业微生物,2016,46(4):14-18,5.
