工业微生物2016，Vol.46Issue(4)：19-24,6.DOI:10.3969/j.issn.1001-6678.2016.04.004
一株耐高温纤维素酶产生菌的分离和鉴定
Isolation and identification of high temperature resistant bacterium for producing cellulase
摘要
Abstract
In order to obtain high temperature resistant strains producing cellulase for the application in the high-temperature industrial cellulose degrading field, filter paper disintegration and CMC-Congo red staining were used to initially screen the cellulose-degrading strains at 45 ℃. 6 target strains were isolated from piles of rice straw in farmland and temporarily marked as A1~A6 . A1 was chosen as experimental strain owning to its higher ratio with 4 of clear circle diameter to colony diameter and higher reducing sugar concentration, which was short rod, G+ and easily fade, motile. Test results such as catalase, amylolysis, gelatin liquefaction, methyl red test, hydrogen sulfide production, glucose fermentation, PHB, metachromatic granule staining, cellulose degradation, denitrification test were positive. Test results such as Voges-Proskauer test, indole test, nitrate reduction test, lecithinase were negative. The enzyme property produced by strain A1 showed its major enzymatic activity was CMC and secondary FPA. According to its enzymatic activity, cell and colony morphology, physiological and biochemical properties, strain A1 was preliminarily classified as genus Cellulomonas.关键词
纤维素酶产生菌/纤维素/耐高温/纤维单胞菌属Key words
cellulase-producing bacterium/cellulose/high temperature resistant/Cellulomonas引用本文复制引用
任世英,邵奎,李雯,张威,刘飞..一株耐高温纤维素酶产生菌的分离和鉴定[J].工业微生物,2016,46(4):19-24,6.基金项目
国家自然科学基金项目(51308245) （51308245）
江苏省淮安市科技计划项目(HAN2015026) （HAN2015026）
江苏省生物质转化与过程集成工程实验室开放课题(JPELBCPL2013004和JPELBCPL2014006)。 （JPELBCPL2013004和JPELBCPL2014006）
