海洋科学2016，Vol.40Issue(6)：62-71,10.DOI:10.11759//hykx20150812003
不同海洋微藻对石油污染的响应差异
Differences in the response of different marine microalgae to oil pollution
摘要
Abstract
Microalgae are a fundamental component of aquatic food web. Oil spills or the release of other sources of hydrocarbons into the marine environments account for most of the organic pollution that occurs in coastal areas. Different species of microalgae will respond differently to oil pollution, producing different downstream effects on phytoplankton communities and possibly reshaping marine ecosystems. Research into the response of different ma-rine microalgae to oil pollution has largely been held back by the high degree of complexity that exists in the rela-tionship between various phytoplankton species and the many different oil pollution scenarios that are possible. The present study uses a high-throughput screening method to examine the responses of different microalgae to oil pol-lution. This is accomplished using oil standard 20-3 and 3 oil chemical products: bisphenol, terephthalic acid, and paraxylene, as sources of oil pollution together with 30 strains of microalgae isolated from the coastal area of China. For the first time, principle component analysis (PCA) based on Simca-P software was used to analyze a large data set measuring population growth of microalgae over 72 h in the presence of various oil pollutants. The resultsshow that the growth of different strains of microalgae was affected differently by the oil pollutants and the effect of oil pollutants was concentration-dependent. Multiwell culture plates, e.g., 24-well plate, were efficient growth vehicles for algae growth when used in the high-throughput screening procedure. Analysis of this large data set showing microalgal population growth in response to various oil pollutants of different concentration can be performedef-fectively by applying the method of PCA based on the computer software Simca-P.关键词
海洋微藻/油污染/种群增殖/大数据Key words
Marine microalgae/oil pollution/population growth/big data分类
生物科学引用本文复制引用
耿沙沙,刘晨,张少宁,周成旭,叶央芳,骆其君,严小军..不同海洋微藻对石油污染的响应差异[J].海洋科学,2016,40(6):62-71,10.基金项目
中国国家科技部星火计划项目(2015GA701001) （2015GA701001）
浙江省自然科学基金资助项目(LY12D06001) （LY12D06001）
国家公益性行业(海洋)科研专项经费项目(201105009) （海洋）
海洋可再生能源专项资金项目(GHME2001SW02) （GHME2001SW02）
国家“万人计划”(财教[2014]412号)@@@@[Foundation：China Spark Program of National Ministry of Science and Technology, No.2015GA701001 （财教[2014]412号）
Natural Science Foundation of Zhejiang Province, No. LY12D06001 （）
National Marine Research Special Funds for Public Welfare Projects, No.201105009 （）
Marine Renewable Energy Spe-cial Project, No. GHME2001SW02 （）
National High-level Personnel of Special Support Program, No.2014-412] （）
