军事医学2016，Vol.40Issue(8)：623-627,5.DOI:10.7644/j.issn.1674-9960.2016.08.002
IL-8通过整合素调控肝癌HepG2细胞迁移
Integrins mediate the migration of HepG2 cells induced by IL-8
摘要
Abstract
[ Abstract] Objective To investigate the effect of IL-8 on the viability and migration of HepG2 cells and the potential effect of integrins (αsubunits ) on the migration of HepG2 cells.Methods HepG2 cells were stimulated by IL-8 at different concentrations ranging from 0 to 125 ng/ml in vitro.MTT assay was preformed to detect the viability of HepG2 cells.Scratch wound migration assay was used to explore the effect of IL-8 on the migration of HepG2 cells at the time points of 0, 4, 8, 12 and 24 h,respectively.Transwell assay was conducted to detect the vertical migration of HepG2 cells after stimulation with IL-8 for 24 h.The F-actin of HepG2 cells was observed by immunofluorescence analysis after treatment with IL-8 at different concentrations.The expression of αsubunits of integrins in HepG2 cells treated with IL-8 for 8h was detected using Western boltting assays.Results Compared with the control group, HepG2 cells treated with IL-8 showed improved proliferation activities, but there was no significant difference between the groups of HepG2 cells treated with IL-8 at different concentrations.The cell scratch healing assays and Transwell analysis both indicated that IL-8 promoted the migration of HepG2 cells in a dose-dependent manner.Meanwhile, the cytoskeleton of HepG2 cells was rearranged and the number of filopodium-like protrusions (FLPs) was increased rapidly after treatment with IL-8.Western blotting analysis showed that IL-8 up-regulated the expression of αsubunit of integrins and there was a concentration difference betweenαsubunits.Conclusion IL-8 promotes the migration of HepG2 cells by up-regulating the expression ofαsubunits, and different αsubunits may play different roles.关键词
肝肿瘤/IL-8/整合素/细胞迁移Key words
liver neoplasms/IL-8/integrin/cell migration分类
医药卫生引用本文复制引用
王毅,杨振华,李雪丹,邱红根,许俊,周法庭..IL-8通过整合素调控肝癌HepG2细胞迁移[J].军事医学,2016,40(8):623-627,5.基金项目
宜昌市卫生科研计划资助项目 （）
