军事医学2016，Vol.40Issue(8)：639-642,4.DOI:10.7644/j.issn.1674-9960.2016.08.005
HPLC法测定放射增敏剂苯并三嗪衍生物YABQ含量及有关物质
Determination of content and related substances of radiosensitizer benzotriazine derivatives YABQ by HPLC
摘要
Abstract
Objective To establish an HPLC method for determination of the content of radiosensitizer YABQ and its related substances.Methods Diamonsil C18 column (250 mm ×4.6 mm,5 μm) was used.The mobile phase consisted of methanol-0.1%formic acid (22∶78) and isocratic elution at a flow rate of 1.0 ml/min.The detection wavelength was 266 nm,the temperature of the column was 30℃, and the injection volume was 10 μl.Results The linear range of YABQ was 7-84 μg/ml, r=0.9992.The detection limit was 8 ng (S/N≥3), and the quantitation limit was 24 ng (S/N≥10). According to destructive sample processing, the separation coefficient between the peaks was above 1.5, indicating that this chromatographic method could meet the YABQ monitoring requirements.Conclusion Method validation and destructive testing show that both the precision and specificity are fine with this method, which could be used for quality control of YABQ and its related substances.关键词
含量测定/辐射增敏药/YABQ/质量控制/高效液相色谱法/苯并三嗪Key words
quantitative analysis/radiation-sensitizing agents/YABQ/quality control/HPLC/benzotriazine分类
化学化工引用本文复制引用
刘少磊,杨建云,肖炳坤,黄荣清..HPLC法测定放射增敏剂苯并三嗪衍生物YABQ含量及有关物质[J].军事医学,2016,40(8):639-642,4.基金项目
国家自然科学基金资助项目（21375147）；国家科技重大新药创制专项资助项目 （）
