检验医学与临床2016，Vol.13Issue(16)：2233-2235,3.DOI:10.3969/j.issn.1672-9455.2016.16.001
赖氨大黄酸对胆汁淤积性肝纤维化大鼠 Smad2、Smad3表达的影响
Effects of rhein lysine on expression of Smad2 and Smad3 in the model of cholestatic rat with liver fibrosis
摘要
Abstract
Objective To observe the effects of rhein lysine on expression of Smad2 and Smad3 in the model of cholestatic rat with liver fibrosis .Methods The model of cholestatic hepatic fibrosis rats was established by ligation of bile duct .The rats were as-signed to the following five groups :control group ,model group ,lysine group ,lower RHL dose group[35 mg/(kg · d)] and higher RHL dose group[70 mg /(kg · d)] .The expression of Smad2 and Smad3 were detected by RT-qPCR and the changes of Smad2 and Smad3 were detected by Western blot .Results RT-qPCR showed that compared with the model group ,the expressions of mRNA of Smad2 and Smad3 in both lower RHL dose group and higher RHL dose group were significantly higher (P< 0 .05) .Western blot showed that compared with model group the expressions in both lower RHL dose group and higher RHL dose group were also sig-nificantly higher (P< 0 .05) .Conclusion RHL can inhibit the activation and proliferation of cholestatic liver fibrosis .And this re-sult may due to the inhibitory effect on conduction of TGF-β1/Smadss signal as well as the protein and mRNA of Smad2 and Smad3 .关键词
胆汁淤积/肝纤维化/赖氨大黄酸/Smad2/Smad3Key words
cholestatic/liver fibrosis/rhein lysine/Smad2/Smad3引用本文复制引用
王琳,郝晓方,张荣花,王密斯,孔艺璇,冯雪妍,章广玲..赖氨大黄酸对胆汁淤积性肝纤维化大鼠 Smad2、Smad3表达的影响[J].检验医学与临床,2016,13(16):2233-2235,3.基金项目
国家自然科学基金资助项目（81201281）；河北省自然科学基金资助项目（H2013209180，C2012401037，H2013209040），河北唐山市科技计划资助项目（12140209A-4）；华北理工大学研究生创新项目（2015S16，2015S17）；国家级、省级大学生创新项目（201410081021）。 （）
