检验医学与临床2016，Vol.13Issue(16)：2256-2257,2260,3.DOI:10.3969/j.issn.1672-9455.2016.16.008
胰岛素放射免疫检测技术外部影响因素分析
Analysis of exterior influential factors of radioimmunoassay on insulin measurement
摘要
Abstract
Objective To investigate the exterior influential factors of radioimmunoassay (RIA ) on insulin measurement . Methods Ten exterior influential factors were established which were emerged frequently in daily work ,including reaction dose , temperature bath time ,centrifuge time ,centrifugal speed ,skills ,properties of samples ,storage condition ,repeated freezing and tha-wing ,calling curve and curve model .So the factors were detected to study whether they influenced the levels of insulin with the method of RIA .Results (1)Among all the factors ,only technical skills had obvious influence on the insulin measurement (P<0 .05) .(2)Together with plasma sample ,long-term preservation with room temperature and repeated freezing and thawing could make the insulin levels decrease significantly (P< 0 .05) .(3)Recalling the standard curve direactly had obvious influence on the lev-el of insulin too(P< 0 .05) ,which can be removed or decreased through quality control curve in some extent .In addition ,different curve models had no significant influence on the insulin measurement (P> 0 .05) .Conclusion In order to remove exterior influen-tial factors on insulin measurement ,firstly ,the standard operation procedure of the experiment should be kept and the operation method should be strengthen ,and then ,suitable sample should be chosen and standard curve should be recalled cautiously ,repeated freezing and thawing should be avoided in our daily work .关键词
胰岛素/放射免疫分析/外部影响因素Key words
insulin/radioimmunoassay/exterior influential factor引用本文复制引用
张巧娣,谢而付,付煜,颜承靖..胰岛素放射免疫检测技术外部影响因素分析[J].检验医学与临床,2016,13(16):2256-2257,2260,3.基金项目
江苏省实验诊断学重点实验室基金（XK201114）；江苏高校优势学科建设工程资助项目（基金编号 JX10231802）。 （）
