临床眼科杂志2016，Vol.24Issue(4)：369-373,5.DOI:10.3969/j.issn.1006-8422.2016.04.027
臭氧对糖尿病大鼠视网膜 SOD、MDA 及 NO的影响
Effects of Ozone enema on retinal levels of SOD, MDA and NO in diabetic rats
摘要
Abstract
Objective To investigate the effects of ozone on retinal levels of SOD, MDA and NO in diabetes rats and to explore an potential treatment for early stage DR.Methods Forty SD rats were used in this study.Animals in con-trol group ( n =10) were fed with regular diet.Other rats ( n =30) were used to create diabetic models induced with STZ.Fasting blood glucose more than 11.1mmol/l at two or more measurements was an indication of successful modeling. Some of the diabetic rats ( n =10) were continually given high sugar and high fat diet for 1 week and then changed to nor-mal diet.Fourteen diabetic rats received ozone enema at 50 μg/kg three times per week for 4 weeks.Animals were then sacrificed and enucleated to collect retina tissue.Results Diabetes were established in totally 24 rats.There was no difference in blood sugar or body weight between diabetic rats that treated with ozone and that not treated with ozone.Reti-nal SOD activity decreased in all diabetic rats when compared with normal rats ( F =6.804, P <0.05).Decreased SOD activity was more profound in untreated diabetic rats than in ozone treated rats but the difference was not significant.MDA increased in all diabetic rats ( F =4.457, P <0.05), and was even higher in rats treated with ozone than in untreated rats ( P <0.05) .NO content was lower in diabetic rats but the change was not significant.However, NO was significantly lower in rats treated with ozone than in normal rats ( P <0.05) .Conclusion Ozone has no effect on blood glucose and body weight in diabetic rats.Ozone can increase SOD activity, mildly decrease MDA in diabetic retina.But its effect on NO is still uncertain.关键词
糖尿病/视网膜病变/臭氧氧化应激Key words
Diabetic retinopathy/Ozone/Oxidative stress引用本文复制引用
谢婷玉,王燕,娄静,陈雪艺..臭氧对糖尿病大鼠视网膜 SOD、MDA 及 NO的影响[J].临床眼科杂志,2016,24(4):369-373,5.基金项目
新疆维吾尔自治区自然科研基金项目 （）
