中南医学科学杂志2016，Vol.44Issue(4)：379-383,389,6.DOI:10.15972/j.cnki.43-1509/r.2016.04.006
FGF-2上调Survivin蛋白的表达拮抗心肌细胞凋亡
FGF-2 Inhibits Cardiomyocytes Apoptosis Induced by Hypoxia/reoxygenation via Upregulating the Expression of Survivin in H9c2 cells
摘要
Abstract
Objective To investigate the mechanism of FGF￣2’ s fighting against H9c2 cardiomyocytes apoptosis in￣duced by Hypoxia/reoxygenation( H/R) . Methods H9c2 cardiomyocytes was employed as the experimental object,cells were incubated by free serum for 24 h for synchronous cell cycle,then,H9c2 cardiomyocytes were randomly divided into 3 groups:Normal group( Control) ,H/R group, H/R+FGF￣2 group. The viability of cardiomyocytes was measured by CCK￣8. The apoptosis rate of cardiomyocytes was detected by Hoechst and Armexin￣V/PI assays with flow cytometry. Survivin ex￣pression were analyzed by western Blot. Results Compared to the Contrd group,H/R significantly decreased the viability of cardiomyocytes and induced cardiomyocytes apoptosis(P<0.01).Treatment with FGF￣2 for H/R significantly increased the viability of cardiomyocytes subjected to H/R,suppressed cardiomyocytes apoptosis induced by H/R and up￣regulated the expression of Survivin ( P<0.05) . Conclusion FGF￣2 inhibits cardiomyocytes apoptosis induced by H/R in H9c2 cells, which the mechanism may be related to the up￣regulation of Survivin expression.关键词
成纤维细胞生长因子-2/细胞凋亡/生存素Key words
fibroblast growth factors-2/apoptosis/survivin分类
医药卫生引用本文复制引用
李国华,危当恒,刘录山,王佐,姜志胜,刘米华,彭娟,肖卫晋,屈顺林,唐之晗,郭芳,彭利军,任重..FGF-2上调Survivin蛋白的表达拮抗心肌细胞凋亡[J].中南医学科学杂志,2016,44(4):379-383,389,6.基金项目
国家自然科学基金项目(81470435、81541005)、湖南省卫生厅科研计划课题( B2011￣041) （81470435、81541005）
