中南医学科学杂志2016，Vol.44Issue(4)：384-389,6.DOI:10.15972/j.cnki.43-1509/r.2016.04.007
体外传代选择对鼠衣原体质粒缺失株培养特性的影响
Effect of In vitro Passage with Selection Pressure on Fitness of Plasmid-free Chlamydia Muridarum Strain CMUT3
摘要
Abstract
Objective To unravel the effect of passage selection pressure on fitness of plasmid￣free Chlamydia muri￣darum strain CMUT3 and uncover the correlation between genetic and phenotypic signatures. Methods CMUT3 organisms were in vitro passaged for 40 generations under the alternating unassisted/assisted￣infection cycle conditions and the infectivity of initial and final generation organisms in cell culture was measured by comparing their attachment efficiency phenotype in the presence or absence of centrifugation.Next￣generation sequencing technology was then used to sequence the genomes of passaged CMUT3G40 and the parental CMUT3G0. Results Passaged CMUT3G40 organisms became less de￣pendent on centrifugation and more efficient for infecting HeLa cells in the absence of centrifugation compared to the paren￣tal CMUT3G0.Comparative genomes analysis of CMUT3G40 and CMUT3G0 revealed that a G to C substitution at position 277313,resulting in a glutamine to glutamic acid amino acid change in the protein product of TC0237 ( 99. 7% in CMUT3G40 versus 0% in CMUT3G0 genomes) . Conclusion The TC0237Q117E missense mutation under in vitro pas￣sage selection pressure can be attributed to an in vitro attachment enhancement phenotype of plasmid￣free C. muridarum CMUT3 organisms.关键词
鼠衣原体/质粒/体外传代/选择压力/下一代测序Key words
Chlamydia muridarum/plasmid/in vitro passage/selection pressure/next generation sequencing分类
医药卫生引用本文复制引用
陈超群,任林,陆春雪,李忠玉,钟光明,吴移谋..体外传代选择对鼠衣原体质粒缺失株培养特性的影响[J].中南医学科学杂志,2016,44(4):384-389,6.基金项目
国家自然科学基金(81072417) （81072417）
