中南医学科学杂志2016，Vol.44Issue(4)：390-394,5.DOI:10.15972/j.cnki.43-1509/r.2016.04.008
1,25-二羟维生素D3对棕榈酸诱导巨噬细胞炎症因子表达的影响
1,25-dihydroxyvitamin D3 Suppresses Palmitic Acid-induced Inflammatory Cytokines Expression in the THP-1 Macrophage
摘要
Abstract
Objective To investigate the effect and mechanism of 1,25￣dihydroxyvitamin D(1,25(OH)2D3) on palmitic acid( PMA)￣induced inflammatory cytokines expression in THP￣1 macrophage. Methods The THP￣1 macropha￣ges were cultured and incubated with 1,25(OH)2D3(10 nmol/L) and/or PMA(250 μmol/L) for 6h.The RT￣PCR and ELISA technology were used to detect mRNA and protein expression of inflammatory cytokines including TNFα,IL￣1β,IL￣6 and IL￣10 in different groups.The THP￣1 macrophages were treated with 1,25(OH)2D3 and/or PMA for 60min.The west￣ernblot technology was used to detect the expression of AMPKα1 and AMPKα1 phosphorylation. Results 1,25( OH) 2 D3 obviously abolished the effect of PMA on the expression of inflammatory cytokines including TNFα, IL￣1β, IL￣6 and in￣creased the expression of IL￣10 in THP￣1 macrophages. 1, 25 ( OH ) 2 D3 significantly promoted the phosphorylation of AMPKα1 in THP￣1 macrophages. Conclusion 1,25(OH)2D3 can suppress PMA￣induced inflammatory cytokines ex￣pression in THP￣1 macrophages via regulation of AMPKα1 phosphorylation.关键词
1,25-二羟维生素D3/棕榈酸/巨噬细胞/炎症反应Key words
1,25-dihydroxyvitamin D/palmitic acid/macrophage/inflammation分类
医药卫生引用本文复制引用
陈雯,朱肖,冯聚玲,李熠,游咏,桂庆军,尹凯..1,25-二羟维生素D3对棕榈酸诱导巨噬细胞炎症因子表达的影响[J].中南医学科学杂志,2016,44(4):390-394,5.基金项目
国家自然科学基金资助项目(81100213,81470569)；南华大学博士启动基金(2015XQD49)；南华大学留学归国人员启动基金(2015XQD55) （81100213,81470569）
