中南医学科学杂志2016，Vol.44Issue(4)：395-399,5.DOI:10.15972/j.cnki.43-1509/r.2016.04.009
PFOS致SH-SY5Y细胞凋亡及可能机制
The Study of SH-SY5Y Cells Apoptosis Induced by PFOS and Its Mechanism
摘要
Abstract
Objective Explore the apoptosis effects of PFOS on SH￣SY5Y cells and its mechanism. Methods The experiment was divided into seven groups,the control (DMSO) and the PFOS groups (1,10,50,100,150,200μmol/L).The cell viability and time￣efficiency of SH￣SY5Y were determined by CCK8,the apoptosis rate were detected by Annex￣in￣V FITC/PI double￣staining,respectively.The effects of PFOS on oxidative stress damage in SH￣SY5Y cells were detected by UV spectrophotometry and fluorescence methods. Results PFOS treatment significantly decreased SH￣SY5Y cell via￣bility in a dose dependent manner. The apoptosis effects were observed in SH￣SY5Y cells obviously after PFOS exposure 48 h;The results of UV and fluorescence spectrophotometry showed that PFOS induced SH￣SY5Y cell superoxide dismutase ( SOD) and glutathione reductase ( GSH) decreased,compared with the control,and the highest group of PFOS decreased GSH from 8±0.12 nmol/mg protein to 5.89±0.9 nmol/mg protein.While the total reactive oxygen species ( ROS) and ma￣londialdehyde (MDA) levels increased,compared with the control,the highest group of PFOS elevated MDA from 1.92± 0. 17 nmol/mg protein to 6.95±0.26 nmol/mg protein. Conclusion PFOS produced oxidative stress damage in SH￣SY5Y cells,which may be the mechanisms of neurotoxicity induced by PFOS.关键词
全氟辛烷磺酸钾盐/成人神经母瘤细胞/氧化应激/凋亡Key words
PFOS/SH-SY5Y cells/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
全小青,李武,曾怀才..PFOS致SH-SY5Y细胞凋亡及可能机制[J].中南医学科学杂志,2016,44(4):395-399,5.基金项目
国家自然科学基金(81273026),湖南省卫生厅( B2011￣044),衡阳市科技局(2011KS9) （81273026）
