中南医学科学杂志2016，Vol.44Issue(4)：409-413,5.DOI:10.15972/j.cnki.43-1509/r.2016.04.012
野黄芩素通过PI3K/Nrf2/HO-1途径减轻LPS诱导的大鼠急性肺损伤
Scutellarein Reduces LPS-induced Acute Lung Injury via Regulation of PI3K/Nrf2/HO-1 in Endotoxemic Rats
王彪 1袁娜 1张永虎 1曾良1
作者信息
- 1. 南华大学附属第二医院急诊科，湖南 衡阳421001
- 折叠
摘要
Abstract
Objective To observe the molecular mechanism of Scutellarein￣induced heme oxygenase 1 ( HO￣1) ex￣pression in endotoxemic acute lung injury rat. Methods Rats were administered LPS ( 30 mg/kg ) by intravenous infusion to induce ALI.Scutellarein (0.1,0.5 and 1.0 mmol/kg) was given 1 h after LPS initiation.Expression of HO￣1 mR￣NA and protein in lungs were detected by real time PCR and Western blot,respectively.The enzymic activity of HO￣1 was detected by colorimetric method;The tissue injury was analyzed by histopathology. Phosphorylation of PI3K and nuclear translocation of Nrf2 were measured by Western blot.The content of TNF￣α,IL￣6 and IL￣10 were measured by ELISA in ser￣um. Results LPS caused a slight HO￣1 induction both at the mRNA and protein level,whereas administration of Scutel￣larein significantly induced higher HO￣1 expression and enzymic activity, as compared with the LPS group. In addition, Scutellarein could also induce PI3K phosphorylation and Nrf2 nuclear translocation.Furthermore,0.5 and 1.0 mmol/kg of Scutellarein could attenuate plasma levels of TNF￣α and IL￣6,and further increased IL￣10 levels compared with the LPS group.However,the beneficial effects of Scutellarein on cytokines expression were reversed by HO￣1 inhibitor SnPP. Con-clusions Scutellarein induces acute lung injury of endotoxemic rat expression of heme oxygenase￣1 by activation of PI3K/Nrf2 pathway.关键词
野黄芩素/急性肺损伤/血红素氧合酶1Key words
Scutellarein/acute lung injury/hemeoxygenase-1分类
医药卫生引用本文复制引用
王彪,袁娜,张永虎,曾良..野黄芩素通过PI3K/Nrf2/HO-1途径减轻LPS诱导的大鼠急性肺损伤[J].中南医学科学杂志,2016,44(4):409-413,5.
