山东农业科学2016，Vol.48Issue(8)：5-9,5.DOI:10.14083/j.issn.1001-4942.2016.08.002
滨麦草特异标记的开发及验证
Development and Validation of Specific Markers of Leymus mollis
摘要
Abstract
Leymus mollis (Trin.)Hara (2n =28,NsNsXmXm)is one of the important genetic resources for wheat genetic improvement,which possesses a variety of excellent antiviral properties such as disease re-sistance and adversity tolerance.In this study,757 pairs of EST -SSR and STS primers were used to amplify the genomic DNA of L.mollis,Psathyrostachys huashanica and wheat varieties China Spring,Huixianhong, Yannong 15 and Jimai 22.And the specific molecular makers were screened for L.mollis,and were used to infer the chromosome -based group home.The results showed that 193 specific markers of L.mollis were screened out including 36 specific markers belonging to Ns group.The results have some practical values and significances to promoting the excellent gene of L.mollis transfer into wheat and breeding new Tritileymus germplasms.关键词
滨麦草/普通小麦/特异分子标记Key words
Leymus mollis/Common wheat/Specific molecular markers分类
农业科技引用本文复制引用
孙文静,王洪刚,李兴锋..滨麦草特异标记的开发及验证[J].山东农业科学,2016,48(8):5-9,5.基金项目
山东省自然科学基金项目（ZR2015CM034）；山东省农业良种工程项目农业生物资源创新利用课题 （）
