生物技术通报2016，Vol.32Issue(8)：90-95,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.08.014
花生 NBS-LRR 类基因的克隆及表达特性分析
Cloning and Expression Pattern Analysis of NBS-LRR Like-gene in Peanut
摘要
Abstract
Based on peanut’s transcriptome and genome data,a full length sequence of NBS-LRR like-gene was cloned. This gene, which showed 79% similarity with disease resistance protein(KHN40407.1)of Glycine soja,was designated as AhNDrp gene. The length of cDNA of AhNDrp gene was 2 832 bp,and contained no intron. AhNDrp gene harbored 5 typical conserved domains with resistance-related protein,and Domain V was repeat sequence with rich leucine. Real-time quantitative PCR analysis showed that AhNDrp gene was expressed exclusively in root,and the expression was significantly increased after aflatoxin treatment,indicating that this gene was involved in the resistance response.关键词
花生/NBS-LRR 基因/基因克隆/表达分析Key words
peanut/NBS-LRR gene/gene clone/expression analysis引用本文复制引用
冯艳芳,耿丽丽,韩榕,张杰..花生 NBS-LRR 类基因的克隆及表达特性分析[J].生物技术通报,2016,32(8):90-95,6.基金项目
国家自然科学基金青年基金项目 （）
