鸭脂肪间充质干细胞分离培养与生物学特性OA北大核心CSCDCSTPCD
Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells
建立禽类脂肪来源的间充质干细胞（adipose-derived stem cells，ADSCs）体外培养体系，探索其生物学特性和多分化潜能。通过酶消化法分离18日龄鸭胚 ADSCs，绘制生长曲线，通过 RT-PCR、免疫荧光和流式细胞术阳性率检测对 ADSCs 进行鉴定，诱导 ADSCs 向成骨细胞和脂肪细胞分化。结果表明，鸭胚胎 ADSCs 体外增殖能力良好；阳性表达 CD29，CD44，CD105，表达率分别为95.39%、94.53%和9…查看全部>>
This study aims to establish in vitro culture system for the poultry adipose-derived stem cells(ADSCs),to study the biological characteristics and the multi-differentiation potential. Enzyme digestion method was applied to separate Beijing duck 18-day-old embryos ADSCs and to draw the growth curve. ADSCs were identified by RT-PCR,immunofluorescence and flow cytometric analysis. Our results suggested that the ADSCs had solid proliferative activity and were po…查看全部>>
刘雪婷;元虹懿;张明海;关伟军
东北林业大学，哈尔滨 150040 中国农业科学院北京畜牧兽医研究所，北京 100193东北林业大学，哈尔滨 150040 中国农业科学院北京畜牧兽医研究所，北京 100193
脂肪间充质干细胞分离培养体外诱导分化多分化潜能生物学特性
adipose-derived stem cellsisolationin vitro directional differentiationmulti-differentiation potentialthe biological characteristics
《生物技术通报》 2016 (8)
鸡原始生殖细胞向精子诱导分化研究
122-128,7
国家自然科学基金项目（31472099），中国农业科学院科技创新工程项目（cxgc-ias-01）
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