生物技术通报2016，Vol.32Issue(8)：177-183,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.08.026
一种浸矿混合菌种的筛选、鉴定及浸矿的研究
Screening and Identification of Mixed Culture,and Its Bioleaching Capacity
摘要
Abstract
It is aimed to screen the highly efficient leaching bacteria from acid mining water of low-grade copper sulfide mine,and also preliminarily investigate the bioleaching capacities of the bacteria. Firstly,a mixed culture FIM-201304 was obtained by 9K enrichment from acid mining water of a low-grade copper sulfide mine in Fujian province. Then,one dominated bacterium D-1 was isolated from the FIM-201304 using 9K solid culture medium. Sequencing technology by high-throughput Illumina Miseq was used to analyze the community structure of FIM-201304. D-1 was identified by phenotypic characteristics and 16S rRNA gene sequencing analysis. Flask experiment was utilized to compare the effects of leaching low-grade copper- and nickel-sulfide between the mixed culture FIM-201304 and a single bacterium D-1. Results from high-throughput sequencing analysis showed that the mixed culture FIM-201304 contained the dominated bacteria of Acidithiobacillus ferrooxidans(Abundance accounted for 85.02%),Pseudomonas sp.(Abundance accounted for 10.07%),Acidiphilium acidophilum(Abundance accounted for 1.30%)and Sphingomonas sp.(Abundance accounted for 1.21%). According to phenotypic characteristics and 16S rRNA gene sequence analysis,strain D-1 was identified as Acidithiobacillus ferrooxidans.The results after 20 days of bioleaching with flasks showed that leaching ratio of copper and nickel by FIM-201304 was 65% and 56% respectively,while the leaching ratio by D-1 was 41% and 36% under the same conditions,i.e.,the bioleaching ratio of copper and nickel by mixed culture increased 24% and 20% compared to that by single bacterium,respectively. Conclusively,the mixed culture resulted in the better leaching effect as heterotrophic bacteria promoted the metal-leaching capacity of autotrophic bacteria from the ore.关键词
嗜酸氧化亚铁硫杆菌/生物浸出/混合菌/高通量测序/16S rRNA 基因Key words
Acidithiobacillus ferrooxidans/bioleaching/mixed culture/high-throughput sequencing/16S rRNA gene引用本文复制引用
聂毅磊,陈宏,罗立津,贾纬,陈星伟..一种浸矿混合菌种的筛选、鉴定及浸矿的研究[J].生物技术通报,2016,32(8):177-183,7.基金项目
福建省科技计划重点项目 （）
