生物技术通报2016，Vol.32Issue(8)：200-206,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.08.029
石斛内生甲基营养芽胞杆菌的拮抗和促生作用研究
Antagonistic Efficacy and Growth-Promoting Effect of Bacillus methylotrophicus Isolated from Dendrobium huoshanense
摘要
Abstract
Isolating endophytic bacteria form Dendrobium huoshanense aimed to obtain endophytic bacteria that have broad-spectrum antimicrobial activity and plant growth-promoting trait. Plate confrontation method was applied to screen the endophytic bacteria with antimicrobial activities against the black spot disease of D. huoshanense,of which the antimicrobial spectrum and plant growth-promoting(PGP) traits were determined. Total 22 endophytes were isolated from D. huoshanense,and strain RA presented strong inhibitory activities against Alternaria tenuissima and it was identified as Bacillus methylotrophicus on the basis of 16S rDNA sequence analysis and was selected for further studies. Strain RA was capable of co-producing IAA,siderophore,protease,biosurfactant,and biofilm,and significantly inhibited 8 plant pathogens and had broad-spectrum antimicrobial effects. A significant improvement in corn seedlings growth was observed when seeds of corn were bacterized prior to sowing. In conclusion,results clearly suggest that strain RA is a potential candidate for use as biocontrol agent and in biofertilizer.关键词
霍山石斛/内生细菌/甲基营养芽胞杆菌/抑菌活性/促生长Key words
Dendrobium huoshanense/endophyte/Bacillus methylotrophicus/antagonistic efficacy/growth promotion引用本文复制引用
武利勤,顾海科,王青,尚宏忠,刘桂君,包放..石斛内生甲基营养芽胞杆菌的拮抗和促生作用研究[J].生物技术通报,2016,32(8):200-206,7.基金项目
北京市科学技术研究院改革与发展专项基金项目 （）
